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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 2814-2820
DOI: https://doi.org/10.20546/ijcmas.2017.612.327


Media Standardization for Hydroponic Culture to Screen Wheat Genotypes for Nitrogen Use Efficiency
K.M. Ranjitha1, Suma S. Biradar2*, S.A. Desai2*, V. Rudra Naik2, Sumangala Bhat1, T.N. Satisha2, Guruprasad Hiremath2, K.J. Yashawantha Kumar2, C.K. Chethana2 and K. Venkatesh3
1Department of Biotechnology, University of Agricultural Sciences,Dharwad 580 005, Karnataka, India
2AICRP on Wheat, MARS, University of Agricultural Sciences, Dharwad 580 005,Karnataka, India
3Indian Institute of Wheat and Barley Research, Karnal, Haryana, India
*Corresponding author
Abstract:

Root architecture is critical for efficient nutrient acquisition. This necessitates the investigations on plant root system to identify genotype with high nitrogen uptake efficiency under target environment. A hydroponic culture was employed to standardize the media to be used for screening of wheat genotypes for Nitrogen Use Efficiency (NUE) using proven low nitrogen susceptible and tolerant genotypes. Four different modified Hoagland solutions (NH4NO3 media, NO3 complete, NO3 half, and N minus media) were used and five wheat genotypes namely, UASBW 11328, GW 322 and PDW 291, NARBADA 4 and C 306 were grown in hydroponic culture for 30 days. Results regarding analysis of variance for interactions of genotypes and nitrogen levels revealed that all the genotypes exhibited the significant difference among themselves for all the four different solutions. Over the different media, low N susceptible genotype C 306 exhibited significant difference for most of the traits. While over the genotypes, both half NO3 and N-minus recorded significant difference for root and shoot traits. Thus it was concluded that, half NO3 medium is the best to screen the wheat genotypes for screening for NUE under 50 per cent supply. Further, this method gives the way for quick initial screening of wheat genotypes for NUE.


Keywords: NUE, Hydroponics, Hogland solution, Variability and Genotype × Nitrogen interaction.
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How to cite this article:

Ranjitha, K.M., Suma S. Biradar, S.A. Desai, V. Rudra Naik, Sumangala Bhat, T.N. Satisha, Guruprasad Hiremath, K.J. Yashawantha Kumar, C.K. Chethana and Venkatesh, K. 2017. Media Standardization for Hydroponic Culture to Screen Wheat Genotypes for Nitrogen Use Efficiency.Int.J.Curr.Microbiol.App.Sci. 6(12): 2814-2820. doi: https://doi.org/10.20546/ijcmas.2017.612.327