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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(6): 563-569
DOI: http://dx.doi.org/10.20546/ijcmas.2016.506.060


Laboratory Diagnosis of Mycobacterium tuberculosis in Resource Constraint Setting: Direct Sputum Smear Microscopy is Comparable with Automated Mycobacterium Growth Indicator Tube (BACTEC MGIT 960)
Ochei, Kingsley Chinedum1, Obeagu, Emmanuel Ifeanyi2, Odo Michael1
and Ugobo Emmanuel Eteng3
1Family Health International (FHI 360) Country Office, Garki Abuja Nigeria
2Diagnostic Laboratory Unit, Health Services Department, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria
3Initiative for People’s Good Health Ugep, Cross River State, Nigeria
*Corresponding author
Abstract:

Accurate diagnostic tests have important role to play in the management of patient and the control of Tuberculosis (TB) infection. Direct microscopy method of diagnosing TB is very common in areas with high prevalence of tuberculosis but the current quest for automated liquid culture like BACTEC Mycobacterium Growth Indicator Tubes (MGIT) 960 has challenge the relevance of direct microscopy. This study aimed to compare direct microscopy and BACTEC MGIT 960 using Lowenstein Jensen culture medium (LJ) as the reference standard. 340 participants were recruited into the study; 192 males and 148 females between the age group 10 to 64 years. Findings shows that BACTEC MGIT 960 has sensitivity and specificity of 100.0% and 56.4% respectively, and a negative predictive value of 100.0% when compared with the reference standard indicating the proportion of AFB negative participants were actually not infected with M. tuberculosis the above test result was compare with the test result from direct microscopy and was statistically significant (P=0.001), indicating that the conventional method is comparable with MGIT. Of the 340 positive sputa by MGIT, 102 specimens were contaminants representing 30% contamination rate. Expensive equipments like BACTEC MGIT 960 for laboratory diagnosis of MTB could be replaced with direct sputum microscopy especially in resource constrain settings.
 


Keywords: Mycobacterium tuberculosis, direct sputum smear microscopy,  automated Mycobacterium Growth Indicator Tube (BACTEC MGIT 960).
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How to cite this article:

Ochei, Kingsley Chinedum, Obeagu, Emmanuel Ifeanyi, Odo Michael and Ugobo Emmanuel Eteng. 2016. Laboratory Diagnosis of Mycobacterium tuberculosis in Resource Constraint Setting: Direct Sputum Smear Microscopy is Comparable with Automated Mycobacterium Growth Indicator Tube (BACTEC MGIT 960).Int.J.Curr.Microbiol.App.Sci. 5(6): 563-569. doi: http://dx.doi.org/10.20546/ijcmas.2016.506.060