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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 2525-2537
DOI: https://doi.org/10.20546/ijcmas.2017.612.293


Intradermal Immunization of BALB/C Mice with Heat Killed Klebsiella pneumoniae ATCC BAA1706 Leads to the Production of both IgG1 and IgG2a
Ítalo Sousa Pereira*, Maria Poliana Leite Galantine, Igor Pereira Ribeiro Muniz, Diego Patrick Soares Lopes, Karine Bitencourt Rodrigues, Lorena Santos Leal, Palloma Porto Almeida, Larissa Rodrigues de Oliveira Sousa and Robson Amaro Augusto da Silva
Universidade Federal da Bahia, Instituto Multidisciplinar em Saúde, Campus Anísio Teixeira, Bahia, Brasil
*Corresponding author
Abstract:

Klebsiella pneumoniae is a gram-negative bacillus commonly isolated from nosocomial pneumonia patients. Given the increasing development of strategies to bypass the therapeutic molecules used today, the development of an immunoprophylactic safe approach against the pathogen is of great clinical relevance. Here we employed the strain of Klebsiella pneumoniae ATCC BAA1706 for the intradermal Immunization of Balb/c mice. Two weeks after three immunizations the mice were intratracheally challenged with 3x108 or 1,5x106 CFUs of K. pneumoniae and euthanized 24h post-infection. We did not detect changes in the peripheral blood cell numbers. However, the infection with 3x108 CFUs of K. pneumoniae led to an increase in the neutrophil count on the bronchoalveolar lavage. The immunization protocol used in this study led to production of IgG serum titers of IgG1 and IgG2a subclasses against the bacterial antigens. Our results exhibited that the intradermal immunization with heat killed K. pneumoniae ATCC BAA1706 led to the activation of the humoral acquired immunity, which has been previously proved participant in K. pneumoniae infections depuration.


Keywords: Klebsiella pneumoniae, Production, Heat.
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How to cite this article:

Ítalo Sousa Pereira, Maria Poliana Leite Galantine, Igor Pereira Ribeiro Muniz, Diego Patrick Soares Lopes, Karine Bitencourt Rodrigues, Lorena Santos Leal, Palloma Porto Almeida, Larissa Rodrigues de Oliveira Sousa and Robson Amaro Augusto da Silva. 2017. Intradermal Immunization of BALB/C Mice with Heat Killed Klebsiella pneumoniae ATCC BAA1706 Leads to the Production of both IgG1 and IgG2a.Int.J.Curr.Microbiol.App.Sci. 6(12): 2525-2537. doi: https://doi.org/10.20546/ijcmas.2017.612.293