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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 2486-2494
DOI: https://doi.org/10.20546/ijcmas.2017.612.289


Comparative Evaluation of Different Phenotypic Methods in Detection of Klebsiella pneumoniae Carbapenemase (KPC)
A. Tejashree* and M. Archana Hegde
Department of Microbiology, JSS Medical College, Mysore, Karnataka, India
*Corresponding author
Abstract:

In recent years, carbapenem resistance has emerged in Klebsiella pneumoniae (K. pneumoniae) isolates due to acquisition of different carbapenemases enzyme. Carbapenemase producing bacteria are often misidentified by routine microbiological susceptibility testing and incorrectly reported as sensitive to carbapenems; however, resistance to the antibiotic ertapenem is common and a better and sensitive indicator of Carbapenem resistance. Routine laboratory detection of carbapenemase producing K. pneumoniae isolates is crucial, both for a therapeutic management and an efficient infection control. The present study was under taken to assess the evaluation of different phenotypic methods in detection of K. pneumoniae Carbapenemase (KPC). All clinical isolates of K. pneumoniae was collected from December 2014 to November 2016 was included in the study. Urine, Pus, ET secretions, BAL, Blood and other Body fluids received in the laboratory were subjected to routine processing as per standard operating procedures. Antimicrobial susceptibility testing was performed as per the CLSI guidelines (2013) by Vitek2 automated system to detect carbapenem resistant K. pneumoniae (CRKP) as primary test. Further, confirmatory test was carried by Modified Hodge test, HiCrome Agar KPC test and Carba NP test as different phenotypic tests. Total of 1,539 Klebsiella pneumoniae were isolated from various clinical samples. Only, 254 (16.50%) isolates were carbapenem resistant K. pneumoniae (CRKP). In which, 184 (72.44%) were positive with clover-leaf indentation for Modified Hodge Test (MHT) followed by 235 (92.51%) were positive for HiCrome KPC agar test and 239 (94.09%) were positive (Color change within 2hrs of incubation from red to orange/yellow color) for Carba NP test. Among the different phenotypic tests conducted, Carba NP test showed 239(94.09%) were positive, which was rapid (<2 hours), inexpensive technique compared to HiChrome agar KPC test and Modified hodge test. This technique can be implemented in any laboratory for early detection of Carbapenem resistance (CR).


Keywords: Carbapenem Resistant-K. pneumoniae, Modified Hodge test, Carba NP test, HiChrome agar KPC test.
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How to cite this article:

Tejashree, A. and Archana Hegde, M. 2017. Comparative Evaluation of Different Phenotypic Methods in Detection of Klebsiella pneumoniae Carbapenemase (KPC).Int.J.Curr.Microbiol.App.Sci. 6(12): 2486-2494. doi: https://doi.org/10.20546/ijcmas.2017.612.289