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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(6): 479-499
DOI: http://dx.doi.org/10.20546/ijcmas.2016.506.055


Analysis of the Effect of Agitation and Aeration on Xylitol Production by Fermentation in Bioreactor with Kluyveromyces marxianus Using Hydrolized Tamarind Seed as Substrate
Ricardo Martínez-Corona1,2, Carlos Cortés Penagos2, María del Carmen Chávez-Parga3, Mariana Alvarez-Navarrete1 and Juan Carlos González-Hernández1*
1Laboratorio de Bioquímica del Departamento de Ing. Bioquímica del Instituto Tecnológico de Morelia, Av. Tecnológico # 1500, Colonia Lomas
de Santiaguito, C. P.  58120, Morelia, Michoacán, México
2Facultad de Químico Farmacobiología de la Universidad Michoacana de San Nicolás de Hidalgo, México
3Posgrado de la Facultad de Ingeniería Química de la Universidad Michoacana de San Nicolás de Hidalgo, México
*Corresponding author
Abstract:

Xylitol is a compound with high industrial value due to its chemical and physical properties; at an industrial level, xylitol is produced by catalytic hydrogenation of xylose, a process both costly and low-yield. As a viable alternative, xylitol can be produced by fermentation with yeasts that use xylose as an energy source, such as Kluyveromyces marxianus. As a substrate source we proposed the use of tamarind seed, with high xylose content in its structure. Making use of xylose by yeast plus the resulting xylitol, depend on various factors among which that of greatest impact is the presence of oxygen in the fermentation medium. Therefore, in this study, the effects of aeration and agitation on xylitol production using hydrolyzed tamarind seed as a substrate medium were evaluated, employing a 22 factorial experimental design with a central point. In this way, aeration and interaction of the factors (both aeration and agitation) were the ones which had a significant effect on xylitol production. The highest yield reached was of 0.71 g of xylitol/g of xylose with agitation at 100 rpm and, aeration at 0.1 vvm in semi anaerobic conditions.
 


Keywords: Xylose, Fermentation, Xylitol, Aeration, Agitation
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How to cite this article:

Ricardo Martínez-Corona, Carlos Cortés Penagos, María del Carmen Chávez-Parga, Mariana Alvarez-Navarrete and Juan Carlos González-Hernández. 2016. Analysis of the Effect of Agitation and Aeration on Xylitol Production by Fermentation in Bioreactor with Kluyveromyces marxianus Using Hydrolized Tamarind Seed as Substrate.Int.J.Curr.Microbiol.App.Sci. 5(6): 479-499. doi: http://dx.doi.org/10.20546/ijcmas.2016.506.055