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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 2254-2269
DOI: https://doi.org/10.20546/ijcmas.2017.612.261


An Insight into Total Soluble Proteins across Rice (Oryza sativa L.) Germplasm Accessions
S.Aiyswaraya1*, R. Saraswathi1, S. Ramchander1, R. Vinoth1, D.Uma2, D. Sudhakar3 and S. Robin1
1Department of Rice, Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore-641003, Tamil Nadu, India
2Department of Plant Biochemistry
3Department of Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University,Coimbatore-641003, Tamil Nadu, India
*Corresponding author
Abstract:

Cereals are the staple foods of mankind and they provide the major portion of the energy, protein and other nutrients in the diets. Protein is an essential component required for growth, antibodies production and immunity in human beings. Screening and evaluation of protein content in 150 rice germplasm accessions was undertaken to identify protein rich germplasm. The germplasm accessions comprised of landraces from different parts of the country and world, CHIR lines, IG lines, ARB lines and RPHP lines. The results of the study revealed that total soluble protein content ranged from 7.54 g/100g to 14.54 g/100g of sample. Among 150 accessions, eight lines had recorded significantly higher protein content (>10.50 g/100g), 48 lines had registered moderate content (9.01 to 10.50 g/100g) and 94 lines had registered low protein content (< 9.00 g/100g). Cluster analysis based on available protein content revealed that, 150 accessions were grouped into two major clusters at the similarity coefficient of 2.24. Cluster I is comprised of three genotypes viz., RG1 (Mapillaisamba), RG7 (Kudaivazhai) and RG110 (Norungan) which recorded higher protein content (14.54 to 12.38g/100g) and these are landraces of Tamil Nadu. Cluster II comprised of 147 accessions that branched into three sub clusters. Sub-cluster 1 had genotypes with high protein content in the range of 10.70 to 10.96 g/100g, while sub cluster II consisted of 48 genotypes with moderate protein content in the range of 9.16 to 10.25g/100g. Finally, 94 genotypes with low protein content (7.54 to 8.71g/100g) fell in sub cluster 3. The high protein genotypes identified in this study can be potentially utilized for mapping of key genomic regions associated with protein content in rice and in biofortification programmes.


Keywords: Rice, Total soluble proteins, Landraces, Germplasm, Clustering.
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How to cite this article:

Aiyswaraya, S., R. Saraswathi, S. Ramchander, R. Vinoth, D. Uma, D. Sudhakar and Robin, S. 2017. An Insight into Total Soluble Proteins across Rice (Oryza sativa L.) Germplasm Accessions.Int.J.Curr.Microbiol.App.Sci. 6(12): 2254-2269. doi: https://doi.org/10.20546/ijcmas.2017.612.261