International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:6, June, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(6): 470-478

Optimization of Protease Production from Bacillus cereus
Kusuma Dorcas1* and Pavan Kumar Pindi2
1Department of Microbiology, Kasturba Gandhi Degree and P.G College for Women West Marredpally, Secunderabad, T.S-500026, India
2Department of Microbiology, Palamuru University, Mahabubnagar, T.S.-509001, India
*Corresponding author

A soil isolate of Bacillus cereus exhibited highest proteolytic activity when grown on casein agar plates with a zone of inhibition of 34mm. The production medium was optimized with respect to physical and chemical factors using submerged fermentation. The optimum pH, temperature and incubation period for protease production were pH 7 at 37°C for 72 hrs at 150rpm. Glucose was found to be an important media component in this. The protease production of this isolate was also studied with respect to the inoculum size, nitrogen sources and metal ions. Ca2+ was found to be a potent enhancer. The extracted crude enzyme showed the ability to remove blood stains and so can be used in detergent industry.

Keywords: Protease production, Bacillus cereus, culture conditions, enzyme activity.

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How to cite this article:

Kusuma Dorcas and Pavan Kumar Pindi. 2016. Optimization of Protease Production from Bacillus cereus.Int.J.Curr.Microbiol.App.Sci. 5(6): 470-478. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.