International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:6, June, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(6): 370-376

Characterization and Cytotoxicity Assay of Pigment Producing Microbes
Kamla Malik1*, Jayanti Tokas2 and Ramesh Chand Anand1
1Department of Microbiology, College of Basic Science & Humanities,
Chaudhary Charan Singh Haryana Agricultural University, Hisar 125004, Haryana, India
2Department of Biochemistry, College of Basic Science & Humanities, Chaudhary Charan Singh Haryana Agricultural University, Hisar 125004, Haryana, India
*Corresponding author

The pigment producing bacteria and fungi were isolated from soil, damping site, compost, industrial effluent, spoiled fruits and vegetables.  A total of fifteen bacterial and nine fungal pigment-producing isolates were obtained and screened on nutrient agar and potato dextrose medium, respectively. The pigments from selected isolates were extracted by solvent extraction (acetone and methanol). The antimicrobial activity of selected isolates was determined by disc agar diffusion technique against standard isolates of Staphylococcus aureus, Klebsiella pneumonia, Escherichia coli and Fusarium oxysporum. The cytotoxicity was studied by using yeast toxicity test (YTT). Bacterial isolates BP-8 exhibited the inhibitory effect against Saccharomyces cerevisiae and E.coli. The Rf value of pigment extract from different bacterial isolates varied from 0.86 to 0.91 whereas it was 0.92 by fungal isolate FP-6 as determined by thin layer chromatography (TLC).  ). The bacterial isolates BP-2 and BP-6 were Gram+ve and BP-8 was Gram-ve. Out of these BP-6 and BP-8 were non spore former. These bacterial isolates belonged to the genus Bacillus, Micrococcus and Serratia. The fungal isolate was identified as Aspergillus sp.

Keywords: Pigment producing bacteria, fungi, extraction, cytotoxicity.

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How to cite this article:

Kamla Malik, Jayanti Tokas and Ramesh Chand Anand. 2016. Characterization and Cytotoxicity Assay of Pigment Producing Microbes.Int.J.Curr.Microbiol.App.Sci. 5(6): 370-376. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.