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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 585-594
DOI: https://doi.org/10.20546/ijcmas.2017.612.071


Molecular Characterization of Erwinia chrysanthemi Causing Soft Rot Disease in Aloe vera
M. Syamala* and C. Ciba
Department of Plant Pathology and Department of Horticulture, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore -641003. Tamil Nadu, India
*Corresponding author
Abstract:

Erwinia chrysanthemi is the causal agent of soft rot of Aloe vera. In the present study, 51 isolates of Erwinia chrysanthemi of Aloe vera from Tamilnadu were characterized. Molecular detection of Erwinia chrysanthemi was carried out using species specific primers and toxin analysis was carried out using PCR and HPTLC analysis. Morphological identifications were well correlated with the molecular detection method. All the 51 isolates expressed an amplified product size of approximately 560 bp and hence confirmed as P. fluorescens. One isolate which expressed an amplified produce approximately 546 bp was confirmed as Bacillus subtilis. The crude antibiotics, viz., phenazine and 2, 4 diacetylphloroglucinol (DAPG) extracted from Pf 32, Pf 45 and Bs5 respectively, were thermostable (1000C) and highly anti-bacterial against E. chrysanthemi. The presence of Phenazine and DAPG was detected through thin layer chromatography (TLC) with an Rf value of 0.57 for the isolates Pf 32, Pf 45 and Pf 4 and for DAPG the Rf value 0.88 was detected for the isolates Pf 32 and Pf 45. Primers Phl2a and Phl2b amplified the predicted size of 745 bp fragment from the genomic DNA of Ph 1 producing Pseudomonas isolates Pf 32 and Pf 45. Primers PCA 2a and PCA 2b amplified the predicted 1,150 bp fragment from genomic DNA of Pseudomonas sp Pf 32 and Pf 45.


Keywords: Aloe vera, Soft rot disease.
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How to cite this article:

Syamala, M. and Ciba, C. 2017. Molecular Characterization of Erwinia chrysanthemi Causing Soft Rot Disease in Aloe vera.Int.J.Curr.Microbiol.App.Sci. 6(12): 585-594. doi: https://doi.org/10.20546/ijcmas.2017.612.071