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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(6): 223-235
DOI: http://dx.doi.org/10.20546/ijcmas.2016.506.026


Bacterial Microbiota Analysis Present in the Nose and Pharynx of a Mexican Young Population
Ana Karina Rodriguez-Vicente1, Jaime Bustos-Martinez2, Dolores Reyes-Duarte3 and Teresita Sainz-Espunes4*
1Doctorado en Ciencias Biológicas y de la Salud. Universidad Autónoma
Metropolitana-Xochimilco. Mexico City, Mexico
2Departamento de Atención a la Salud. Universidad Autónoma Metropolitana-Xochimilco, Mexico City, Mexico
3Departamento de Procesos y Tecnología. Universidad Autónoma
Metropolitana-Cuajimalpa, Mexico City, Mexico
4Departamento de Sistemas Biológicos. Universidad Autónoma Metropolitana-Xochimilco, Mexico City, Mexico
*Corresponding author
Abstract:

Culture-independent microbiota is relatively unexplored in Mexican population. The aim of this study was to characterize the microbiota of Mexican young healthy adults by means of traditional culture and metagenomic analysis in order to provide novel insights and have a better understanding about the healthy baselines from which to detect differences associated with diseases. The bacterial microbiota of the nose and pharynx from 75 healthy nonsmoking Mexican young adults was examined by conventional cultures and culture-independent methods. The hypervariable region (V6-V8) of the16S rRNA gene was PCR amplified from isolated DNA and DGGE analyzed, bands excised were sequenced and phylogenetic analysis was done. The study showed that the bacterial microbiota of the pharynx was richer than that of the nose. Using conventional culture methods results showed that gram positive Staphylococcus aureus and S. epidermidis were found in both niches and gram negative bacteria such as Escherichia coli, Klebsiella sp. and Moraxella sp. were the most abundant genera in the nose and Enterobacter sp. in the pharynx. Firmicutes and Proteobacteria phyla accounted for the majority of the bacteria detected in both niches. To our knowledge this is the first report describing partially the composition of and the variability within the nasopharyngeal microbiota of a Mexican  young adult population and therefore we will be able to investigate other different cities and compared them to find out where are the highest microbial contaminated zones and take some hygienic measures in accordance to the normativity reports.


Keywords: Bacterial Microbiota, Nose and Pharynx, metagenomic analysis.
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How to cite this article:

Ana Karina Rodriguez-Vicente, Jaime Bustos-Martinez, Dolores Reyes-Duarte and Teresita Sainz-Espunes. 2016. Bacterial Microbiota Analysis Present in the Nose and Pharynx of a Mexican Young Population.Int.J.Curr.Microbiol.App.Sci. 5(6): 223-235. doi: http://dx.doi.org/10.20546/ijcmas.2016.506.026