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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 4201-4210
DOI: https://doi.org/10.20546/ijcmas.2017.611.492


Evaluation of Different Phenotypic Methods Versus Polymerase Chain Reaction for Detection of Plasmid Mediated AmpC β-Lactamase-Producing Strains of Proteus mirabilis
Rania A. Amar1 and Karim A. Montasser2*
1Department of Clinical and Chemical Pathology, Faculty of Medicine,Ain Shams University, Cairo, Egypt
2Department of Clinical and Chemical Pathology, Faculty of Medicine,
Helwan University, Cairo, Egypt
*Corresponding author
Abstract:

In recent years, the prevalence of infections with multidrug resistant Enterobacteriaceae has steadily increased. Enterobacteriaceae producing AmpC β-lactamases (AmpCs) have become a major therapeutic challenge. The detection of AmpC-producing Proteus mirabilis is of significant clinical relevance, as this may lead to inappropriate antimicrobial regimens and therapeutic failure. The aim of this study is to evaluate and comparing routinely phenotypic methods in detection of resistance with molecular methods. From this study, it can be concluded that cephamycin-Hodge test is the most sensitive, specific, interpretable and efficient test for detection of AmpC β-lactamases in clinical isolates of Proteus mirabilis, compared to the molecular method.


Keywords: Plasmid mediated AmpCβ lactamases, Proteus Mirabilis, Cephamycin-Hodge test, Tris-EDTA disk test, Combination disk test, PCR.
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How to cite this article:

Rania A. Amar and Karim A. Montasser. 2017. Evaluation of Different Phenotypic Methods Versus Polymerase Chain Reaction for Detection of Plasmid Mediated AmpC β-Lactamase-Producing Strains of Proteus mirabilis.Int.J.Curr.Microbiol.App.Sci. 6(11): 4201-4210. doi: https://doi.org/10.20546/ijcmas.2017.611.492