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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(6): 179-189
DOI: http://dx.doi.org/10.20546/ijcmas.2016.506.022


Identification of New Genes Involved in mtDNA Maintenance in Caenorhabditis elegans that could Represent Candidate Genes for Mitochondrial Diseases
Matthew Glover Addo1,2*, Raynald Cossard1, Damien Pichard1, Kwasi Obiri-Danso2, Agnes Rötig3 and Agnes Delahodde1
1Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ Paris-Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette cedex, France
2Department of Theoretical and Applied Biology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
3INSERM UMR 1163, Laboratory of Genetics of mitochondrial disorders, Paris Descartes – Sorbonne Paris Cité University, Imagine Institute, 24 Boulevard du Montparnasse, Paris 75015, France
*Corresponding author
Abstract:

Identification of genes involved in mtDNA maintenance in Caenorhabditis elegans that could represent candidate genes for mitochondrial diseases associated with quantitative or qualitative mtDNA abnormalities was performed using our previous validated method of RNAi combined with ethidium bromide. This was to knock down C. elegans genes homologous to human genes known to be involved in mtDNA stability. Silencing of these candidate genes were repeated again without EtBr to measure the worm’s mtDNA content and the efficiency of gene silencing by Q-PCR and RT-Q-PCR. Among the 182 genes encoding mitochondrial proteins tested randomly, we found that inactivation of 25 of them lead to EtBr sensitivity. Out of these genes, 20 fall into four main categories of metabolism (GOT2, WWOX, ALDH1L2, WWOX, AGMAT and ACOT9), protein synthesis (TRMU, MRPL47, MRPS14, MRPS18C, MRPL4 and MRPL12), mitochondrial morphology (OPA1,) and respiratory chain oxidative phosphorylation (ATP5F1, NDUFS4, FH, CYP2C8, CYP3A7, CYP2C8 and CYP2C8). The human orthologs of these genes as presented in this study could be considered as reservoir of new genes which will represent a helpful tool and knowledge for mitochondrial diseases, as they would be considered as candidate genes for patients.


Keywords: candidate genes, mtDNA, EtBr, orthologs, mitochondrial, diseases.
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How to cite this article:

Matthew Glover Addo, Raynald Cossard, Damien Pichard, Kwasi Obiri-Danso, Agnes Rötig and Agnes Delahodde. 2016. Identification of New Genes Involved in mtDNA Maintenance in Caenorhabditis elegans that could Represent Candidate Genes for Mitochondrial Diseases.Int.J.Curr.Microbiol.App.Sci. 5(6): 179-189. doi: http://dx.doi.org/10.20546/ijcmas.2016.506.022