International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:6, June, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(6): 144-149

DNA Quantification Studies of Selaginella bryopteris (L.) Bak. Genotypes in Telangana, India
P.Rupa* and N. Lakshmi Bhavani
Plant Tissue Culture and Molecular Genetics Lab, Department of Botany, University College of Science, Saifabad, Osmania University, Hyderabad 500004, India
*Corresponding author

Selaginella bryopteris (L.)Bak. is a resurrection plant with unique desiccation tolerance character. It is lithophytic, heterosporous, first vascular and potent medicinal herb belonging to the family Selaginellaceae. Selaginella bryopteris (L.) Bak. genotypes were identified, collected and preserved from various hilly regions of Rangareddy, Medak, Mahaboobnagar, Karimnagar and Warangal districts of Telangana, India. Collected plant genotypes were used to screen the genomic DNA, quantification and their analysis. For the DNA isolation modified CTAB method of Murray and Thomson was used. Quantity and quality of genomic DNA of Selaginella bryopteris (L.)Bak. genotypes were assessed by spectrophotometry and gel electrophoresis methods. The range of DNA Quantification values (251 ng/µl to 879ng/µl) and DNA Purity index values (1.13 to 2.37) of Selaginella bryopteris (L.) Bak. indicated the diversity among genotypes.

Keywords: Selaginella bryopteris (L.) Bak., CTAB method, DNA isolation and Telangana.

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How to cite this article:

Rupa, P.  and Lakshmi Bhavani, N. 2016. DNA Quantification Studies of Selaginella bryopteris (L.) Bak. Genotypes in Telangana, India.Int.J.Curr.Microbiol.App.Sci. 5(6): 144-149. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.