International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:6, June, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(6): 125-129

Evaluation of a Cold Staining Method for Detecting Acid Fast Bacilli in the Sputum
Kiran Yadav*, Gunjan Dutta and Jugal Kishor Aggarwal
Saraswathi Institute of Medical SciencesHapur, Uttar Pradesh, India
*Corresponding author

Microscopic examination of sputum is the cornerstone in diagnosis of pulmonary tuberculosis in developing world, which usually done by Ziehl-Neelsen (ZN) method. However, due to limited laboratory facilities this procedure appears to be adversely affected in field conditions and at peripheral health institutions. Hence, it has become necessary to look for a procedure, which can be used as alternative in such conditions. We evaluated cold staining method for detection of acid fast bacilli in sputum smears. Total 587consecutive sputum samples both spot and early morning from 300 patients were collected and were stained with both Z-N stain and cold AFB stain and results were compared. Out of 300 patients, a total of 58 (19.3%) were AFB positive by Z-N stain and 55 (18.3%) were positive by cold staining method.  By comparing the two we get sensitivity of 94.83%, Specificity of 100%; Positive predictive value of 100%, and Negative predictive value of 98.78% by cold staining method against Z-N stain.

Keywords: Acid fast bacilli, Cold stain, Tuberculosis, Ziehl Nelseen stain.

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How to cite this article:

Kiran Yadav, Gunjan Dutta and Jugal Kishor Aggarwal. 2016. Evaluation of a Cold Staining Method for Detecting Acid Fast Bacilli in the Sputum.Int.J.Curr.Microbiol.App.Sci. 5(6): 125-129. doi:
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