National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Various mutated forms of β-lactamases pose a therapeutic challenge to the health care settings as they hydrolyze a wide range of β-lactam antibiotics. Detection of these enzymes and their co existence is still a diagnostic challenge. In this, we evaluated a novel 12 disc test as a screening test for the presence of any kind of beta lactamases or its co producers. A 12 disc test was put on 300 isolates that showed susceptibility pattern as Intermediate/Resistant to ceftazidime/ ceftriaxone or aztreonam on routine sensitivity testing by modified Kirby bauer method. Further, double disc synergy test, AmpC disc test, E test for MBL and modified Hodge test for KPC/ MBL (used as confirmatory tests) were also done on each of them. By 12 disc test, out of 300 isolates, 64 (21.3%) were ESβL, 8 (2.6%) K1βL, 76 (25.3%) high level AmpCβL, 08(2.6%) AmpCβL, 08 (2.6%) AmpCβL+ ESβL co producers, 108 (36%) Carbepenamases and 28 (9.3%) MβL+ ESβL producers. By confirmatory tests, 64 (21.3%) were ESβL, 08 (2.6%) AmpCβL, 64 (21.3%) High level AmpCβL, 08 (2.6%) AmpCβL + ESβL, 44 (14.6%) AmpCβL + MβL, 16 (5.3%) AmpCβL+ MβL+ ESβL, 52 (17.3%) MβL, 8 (2.6%) KPC and 12 (4%) ESβL+ MβL. 12 disc test is a good screening test for rapid identification of type of β lactamases.
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