International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:6, June, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(6): 105-114

Evaluation of a 12 Disc Test for Phenotypic Detection of β- lactamases Resistance in Gram Negative Bacilli
Parul Sinha, Pallawi Goyal, Rajni Sharma*, Aruna Vyas and R.K. Maheshwari
Department of Microbiology and Immunology, Sawai Man Singh Medical
College, Jaipur, India
*Corresponding author

Various mutated forms of β-lactamases pose a therapeutic challenge to the health care settings as they hydrolyze a wide range of β-lactam antibiotics. Detection of these enzymes and their co existence is still a diagnostic challenge. In this, we evaluated a novel 12 disc test as a screening test for the presence of any kind of beta lactamases or its co producers. A 12 disc test was put on 300 isolates that showed susceptibility pattern as Intermediate/Resistant to ceftazidime/ ceftriaxone or aztreonam on routine sensitivity testing by modified Kirby bauer method. Further, double disc synergy test, AmpC disc test, E test for MBL and modified Hodge test for KPC/ MBL (used as confirmatory tests) were also done on each of them. By 12 disc test, out of 300 isolates, 64 (21.3%) were ESβL, 8 (2.6%) K1βL, 76 (25.3%) high level AmpCβL, 08(2.6%) AmpCβL, 08 (2.6%) AmpCβL+ ESβL co producers, 108 (36%) Carbepenamases and 28 (9.3%) MβL+ ESβL producers. By confirmatory tests, 64 (21.3%) were ESβL, 08 (2.6%) AmpCβL, 64 (21.3%) High level AmpCβL, 08 (2.6%) AmpCβL + ESβL, 44 (14.6%) AmpCβL + MβL, 16 (5.3%) AmpCβL+  MβL+ ESβL, 52 (17.3%) MβL, 8 (2.6%) KPC and 12 (4%) ESβL+ MβL. 12 disc test is a good screening test for rapid identification of type of β lactamases.

Keywords: 12 disc test, beta lactamases, AmpCβL, co producers.

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How to cite this article:

Parul Sinha, Pallawi Goyal, Rajni Sharma, Aruna Vyas and Maheshwari, R.K. 2016. Evaluation of a 12 Disc Test for Phenotypic Detection of β- lactamases Resistance in Gram Negative Bacilli.Int.J.Curr.Microbiol.App.Sci. 5(6): 105-114. doi:
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