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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 3300-3313
DOI: https://doi.org/10.20546/ijcmas.2017.611.387


Expression of Defense Responses in Tomato against Vascular Wilt and Fruit Borer through Consortia of Fluorescent Pseudomonads and Entomopathogenic Fungi
P. Lakshmidevi1*, S. Parthasarathy1, C. Gopalakrishnan1, G. Karthikeyan2, S. Sridharan2 and T. Saraswathi3
1Department of Plant Pathology
2Department of Agricultural Entomology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore–641003, India
3Department of Vegetable Crops, Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore–641003, India
*Corresponding author
Abstract:

Tomato (Solanum lycopersicum L.) is a major contributor to the fruit vegetable diet of humans. It is cultivated in essentially all countries either in fields or in protected culture. Tomato crop is affected by various pest and diseases. Among the diseases, Helicoverpa fruit borer and Fusarium vascular wilt is regarded as the most devastating to tomato around the world. The biological control method has been considered as a promising approach for the management of pest and diseases by enhancing the inherent systemic resistance. However the most of the approaches mainly biological control of pest and disease have used a single organism. This accounts for inconsistency in the antagonistic activity of biocontrol agent. So the combinatorial application of biocontrol agents could be viable for the management major pests and diseases in crop plants. In our study, both Pseudomonas fluorescens and Beauvaria bassiana possess excellent antagonistic activity against F. oxysporum f.sp. lycopersici and H. armigera respectively under in vitro. Combinatorial application of P. fluorescens and B. bassiana was found to reduce the pest and pathogen in tomato crop under glass house condition. Also, which imparts innate immune response by the production of defence related enzymes viz., peroxidase, polyphenol oxidase, superoxide dismutase and catalase on the treated host against necrotrophic pathogen and herbivore. The native polyacrylamide gel electrophoresis (PAGE) experiment showed that one to four isoforms of the defense related enzymes each with greater intensity were expressed in the given treatments. This is indicating that the restriction of insect pest and pathogens in tomato plants was mainly due to application of microbial bio-consortia.


Keywords: Biological control, Fusarium wilt, Helicoverpaarmigera, Pseudomonas fluorescens, Tomato.
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How to cite this article:

Lakshmidevi, P., S. Parthasarathy, C. Gopalakrishnan, G. Karthikeyan, S. Sridharan and Saraswathi, T. 2017. Expression of Defense Responses in Tomato against Vascular Wilt and Fruit Borer through Consortia of Fluorescent Pseudomonads and Entomopathogenic Fungi.Int.J.Curr.Microbiol.App.Sci. 6(11): 3300-3313. doi: https://doi.org/10.20546/ijcmas.2017.611.387