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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:11, November, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 3286-3293
DOI: https://doi.org/10.20546/ijcmas.2017.611.385


A Novel Method to Develop Paste Formulation of Trichoderma harzianum
S. Balakrishnan*, S. Parthasarathy, A. Kamalakannan, K.SenthilKuppusamy and C. Gopalakrishnan
Department of Plant Pathology, Center for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore– 641003, Tamil Nadu, India
*Corresponding author
Abstract:

Biological control of diseases has been practiced as an alternative to control of plant disease using fungicides. Trichoderma spp. is the most widely exploited antagonistic fungi for the management of plant diseases world-wide. Though the biological control of plant diseases using Trichoderma spp. has been impressive and consistence under in vitro conditions, numbers of reports indicate wide variations in the disease control efficiency under field conditions. Formulation plays a significant role in determining the final efficacy of biocontrol agents. Several reports have emphasized the development of suitable formulations for the management of various plant diseases. The present study was proposed to develop a formulation of T. harzianum suitable for application through drip irrigation with longer shelf life. Among the 8 media tested, medium – M3 has recorded the maximum mycelial dry weight of 654 mg. The highest population (11.6×1010cfu/g) was recorded in the fresh formulation containing 10 per cent starch + copper sulphate 20 ppm at pH 4. Starch 10 per cent and copper sulphate20 ppm adjusted to pH 4 has recorded significantly higher population even after 120 days of storage.


Keywords: Copper sulphate, Molasses, Paste Formulation, Starch, Trichoderma harzianum

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How to cite this article:

Balakrishnan, S., S. Parthasarathy, A. Kamalakannan, K. SenthilKuppusamy and Gopalakrishnan, C. 2017. A Novel Method to Develop Paste Formulation of Trichoderma harzianum.Int.J.Curr.Microbiol.App.Sci. 6(11): 3286-3293. doi: https://doi.org/10.20546/ijcmas.2017.611.385
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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