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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 3058-3065
DOI: https://doi.org/10.20546/ijcmas.2017.611.358


Screening, Isolation and Evaluation of Secondary Metabolite Producing Streptomyces against Spider Mite, Tetranychus urticae on Okra
Rajesh Ramasamy*, Jaivel Nanjundan and Marimuthu Ponnusamy
Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, India
*Corresponding author
Abstract:

A survey was conducted in Tamil Nadu and Maharashtra to isolate the actinomycetes from the soil samples for study. Twenty seven cultures of actinomycetes were isolated, purified and designated as actinomycetes (A1 to A27). The test cultures A1 to A27 were taken for studying their acaridical activity. Preliminary screening revealed that five of the isolates were active against spider mite, Tetranychus urticae on okra. Out of the sixteen isolates A7 and A27 isolate showed highest activity against red spider mite, Tetranychus urticae. The elite isolate A7 and A27 was selected for further study. From the isolates only A7 showed maximum antimicrobial activity. This A7 isolate was selected and used for the present study to control red spider mite. Based on the mycelial and cellular morphology of A7 isolate observed under phase contrast microscope (100X), the actinomycete isolate was identified as Streptomyces fradiae. The 16s r DNA gene sequence A7 was found to be 98 per cent similar to S. fradiae. The 16s r DNA gene sequence A27 was found to be 98 per cent similar to S. fradiae. The phylogenetic tree obtained by applying the neighbour-joining method.


Keywords: Screening, Isolation and evaluation, Secondary metabolite.
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How to cite this article:

Rajesh Ramasamy, Jaivel Nanjundan and Marimuthu Ponnusamy. 2017. Screening, Isolation and Evaluation of Secondary Metabolite Producing Streptomyces against Spider Mite, Tetranychus urticae on Okra.Int.J.Curr.Microbiol.App.Sci. 6(11): 3058-3065. doi: https://doi.org/10.20546/ijcmas.2017.611.358