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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 2763-2772
DOI: https://doi.org/10.20546/ijcmas.2017.611.327


Comparative Study of High Quality Genomic DNA Extraction Protocols in Rice and Tomato Crops
Shiv Mani Dubey, Sujeet Kumar*, S.H. Antre and P.H. Ramanjini Gowda
Department of Plant Biotechnology, University of Agricultural Sciences, GKVK, Bengaluru-560065, Karnataka, India
*Corresponding author
Abstract:

This study presents a rapid efficient DNA extraction protocol for rice and tomato crops. Five published protocols (Doyle & Doyle CTAB Procedure, Urea Method, Salt extraction method, IRRI Method, Dellaporta DNA Extraction method) were comparatively evaluated in rice and tomato crops for DNA purity and yield. The analysis of variance revealed that the yield was significantly different (P < 0.01) for all protocols in both crops, whereas there was no significant observation was observed for purity. The multiple Duncan test revealed that, Doyle & Doyle CTAB Procedure was best for rice crop (2481 ng/µl), whereas urea method was best for tomato crop (2685 ng/µl). Here Proposed that urea method, Doyle & Doyle CTAB Procedure is best method for good quality DNA and higher yield in tomato and rice crop respectively. Hence this method of extraction would be ideal for genotyping of large populations of rice and tomato genotypes.


Keywords: Oryza sativa, Solanum lycopersicum, DNA extraction, Genomic DNA, DNA purity.
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How to cite this article:

Shiv Mani Dubey, Sujeet Kumar, S.H. Antre and Ramanjini Gowda, P.H. 2017. Comparative Study of High Quality Genomic DNA Extraction Protocols in Rice and Tomato Crops.Int.J.Curr.Microbiol.App.Sci. 6(11): 2763-2772. doi: https://doi.org/10.20546/ijcmas.2017.611.327