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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 2726-2731
DOI: https://doi.org/10.20546/ijcmas.2017.611.321


Effect of Different Growth Regulators on Soybean (Glycine max L.) Regeneration
B.U. Rathod1*, N.R. Dattagonde2, M.P. Jadhav1 and P.P. Behre2
1Department of Biotechnology, University of Agricultural Sciences, Dharwad, Karnataka, India
2Department of Crop Science, Mahatma Gandhi College of Agricultural Biotechnology,
Nanded, Maharashtra, India
*Corresponding author
Abstract:

In present study efforts were made to improve the regeneration protocol in the popular local soybean cultivars MAUS-71 and MAUS-162. Embryonic axis and Cotyladory node explants were taken and inoculated on different concentration of 2,4-D. Among those MS6D media containing 6mg/L 2,4-D were give best response for callus initiation. After proper callus formation these explants were transferred to shoot initiation and multiplication medium containing different concentration of BAP. MS media fortified with 3 mg/L BAP (MS3B) shows maximum shoot initiation and multiplication results. The Cotyladory node explants of cv. MAUS-71 shows maximum growth and development in all three types of media for all types growth conditions. The MS media fortified with 0.3 mg/L IBA as growth regulators gives better results for root formation in both types of explants and in both cultivars.


Keywords: Soybean, Regeneration, 2,4-D, BAP, IBA.
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How to cite this article:

Rathod, B.U., N.R. Dattagonde, M.P. Jadhav and Behre, P.P. 2017. Effect of Different Growth Regulators on Soybean (Glycine max L.) Regeneration.Int.J.Curr.Microbiol.App.Sci. 6(11): 2726-2731. doi: https://doi.org/10.20546/ijcmas.2017.611.321