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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 2676-2685
DOI: https://doi.org/10.20546/ijcmas.2017.611.315


Detection of Rutin, Kaepferol, and Quercetin based Crude from Corn Silk and Studying their Effects on the Inhibition of Pure Urease Enzyme and Urease of Klebsiella Species
Rafah H. Ismael1*, Salman A. Ahmed1 and Suhad S. Mahmoud2
1Department of chemistry, Collage of Science, Al-Nahrain University, Baghdad, Iraq
2Department of Biotechnology, College of Science, University of Baghdad, Baghdad, Iraq
*Corresponding author
Abstract:

This paper was carried out for detection of Rutin, Kaepferol, and Quercetin in corn silk, and an investigation of their effects (within crude) on the inhibition of urease enzyme and Klebsiella species urease. An HPLC has been used to detect the three flavonoid components in three extracts of corn silk (using 99.9% ethanol, 80% ethanol and water). The results were (0.012mg/L) of Quercetin in 99.9% ethanolic extract (99.9% EE). (0.1398 mg/L), (0.15 mg/L), and (0.11 mg/L) of Rutin, Kaepferol, and Quercetin respectively in 80% ethanolic extract (80% EE). (0.071 mg/L) and (0.091 mg/L) of Rutin and Kaepferol respectively in aqueous extract (AE). It is noticed that only 80% ethanol has an ability to extract such three flavonoids. All such extracts revealed an effective inhibition of urease enzyme in the Klebsiella species with (IC50 = 77.06, 13.54 +- and 35.93 mg/L) for 99.9% ethanol, 80% ethanol and aqueous extracts respectively, while standard urease inhibitor exhibits (IC50 = 453.4 mg/L) for thiourea (TU) as an example. Lastly, such extracts were utilized in the inhibition of pure enzyme and they exhibited an efficient inhibition of (IC50 = 402.8, 95.8, and 348 mg/L) for 99.9% ethanol, 80% ethanol and aqueous extracts respectively as compared with standard urease inhibitor of which gives (IC50 = 54077 mg/L) in thiourea (UT).


Keywords: Ethanol, Flavonoid, Urease inhibition, HPLC, Rutin, Quercetin, Kaepferol.
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How to cite this article:

Rafah H. Ismael, Salman A. Ahmed and Suhad S. Mahmoud. 2017. Detection of Rutin, Kaepferol, and Quercetin based Crude from Corn Silk and Studying their Effects on the Inhibition of Pure Urease Enzyme and Urease of Klebsiella Species.Int.J.Curr.Microbiol.App.Sci. 6(11): 2676-2685. doi: https://doi.org/10.20546/ijcmas.2017.611.315