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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(6): 32-41
DOI: http://dx.doi.org/10.20546/ijcmas.2016.506.004


Isolation and Characterization of Bacteria and Yeast from Kombucha Tea
Tehmeena Ashraf Mukadam*, Kapil Punjabi, Sunita D Deshpande,Shashikant Prabhakar Vaidya and Abhay Shadashiv Chowdhary
Clinical Pathology Department, Haffkine Institute for Training, Research and Testing,Acharya, Donde Marg, Parel, Mumbai, India
*Corresponding author
Abstract:

Kombucha is a sugared tea fermented with flat, pancake like culture of yeast and acetic acid bacteria (AAB), which is consumed worldwide for its refreshing and beneficial effects. In this study Kombucha culture from a fermented tea was screened for the presence of AAB and yeast using selective media. Biochemical analysis for identification and confirmation of the genus of the bacteria and yeast were performed, followed by molecular characterization using partial sequencing targeting conserved sequences of both organisms. Biochemical analysis confirmed presence of organisms belonging to genus Acetobacter. Yeast was unidentified by biochemical analysis. Molecular characterization of the isolates identified acetic acid bacteria as Komagataeibacter saccharivorans and yeast as Zygosaccharomysis bailli.


Keywords: Acetobacter, Kombucha,lichen, yeast, Acetic acid bacteria.
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How to cite this article:

Tehmeena Ashraf Mukadam, Kapil Punjabi, Sunita D Deshpande, Shashikant Prabhakar Vaidya and Abhay Shadashiv Chowdhary. 2016. Isolation and Characterization of Bacteria and Yeast from Kombucha Tea.Int.J.Curr.Microbiol.App.Sci. 5(6): 32-41. doi: http://dx.doi.org/10.20546/ijcmas.2016.506.004