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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 1435-1444
DOI: https://doi.org/10.20546/ijcmas.2017.611.171


Effect of Soybean Leaf Protease Inhibitor on the Mean Leaf Area Consumed by Spodopetra litura and Spilosoma obliqua larvae
Anchala Nautiyal*, Neeta Gaur and Kamendra Singh
Department of Entomology, College of Agriculture, G.B. Pant University of Agriculture and Technology, Pantnagar-263145, U.S. Nagar, Uttarakhand, India
*Corresponding author
Abstract:

The aim of the present investigation was to study the effect of soybean leaf protein and protease inhibitor content on the mean leaf area consumed by Spodoptera litura and Spilosoma obliqua larva. The activity of trypsin inhibitor was assayed by determining the residual trypsin activity by using BapNA as the substrate and bovine trypsin as the standard enzyme. The highest and lowest TIA activity exhibited by genotypes SL 979 and SL 688 (2.25 TUI.mg-1protein) and CSB 904 (0.35 TUI.mg-1protein), respectively. The highest gut trypsin inhibition activity ascertained in larvae fed on SL 688 (14.40 %) While the lowest intensity of gut trypsin inhibition activity was observed in genotypes NRC 94 (0.12%). A highly significant and negative correlation was observed between MLAC (cm2) by S. litura and S. obliqua and protein content in 33, leaves (r= -0.728**) and (-0.674**) respectively and trypsin inhibitor in leaves (r= -0.909**) and (r= -0.913**). Thus it can be concluded that the genotypes which were having higher protein and trypsin inhibitor in their leaves offered resistance against S. litura and S. boliqua in soybean.


Keywords: Spodopetra litura and Spilosoma obliqua larvae.
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How to cite this article:

Anchala Nautiyal, Neeta Gaur and Kamendra Singh. 2017. Effect of Soybean Leaf Protease Inhibitor on the Mean Leaf Area Consumed by Spodopetra litura and Spilosoma obliqua larvae.Int.J.Curr.Microbiol.App.Sci. 6(11): 1435-1444. doi: https://doi.org/10.20546/ijcmas.2017.611.171