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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:11, November, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 1435-1444
DOI: https://doi.org/10.20546/ijcmas.2017.611.171


Effect of Soybean Leaf Protease Inhibitor on the Mean Leaf Area Consumed by Spodopetra litura and Spilosoma obliqua larvae
Anchala Nautiyal*, Neeta Gaur and Kamendra Singh
Department of Entomology, College of Agriculture, G.B. Pant University of Agriculture and Technology, Pantnagar-263145, U.S. Nagar, Uttarakhand, India
*Corresponding author
Abstract:

The aim of the present investigation was to study the effect of soybean leaf protein and protease inhibitor content on the mean leaf area consumed by Spodoptera litura and Spilosoma obliqua larva. The activity of trypsin inhibitor was assayed by determining the residual trypsin activity by using BapNA as the substrate and bovine trypsin as the standard enzyme. The highest and lowest TIA activity exhibited by genotypes SL 979 and SL 688 (2.25 TUI.mg-1protein) and CSB 904 (0.35 TUI.mg-1protein), respectively. The highest gut trypsin inhibition activity ascertained in larvae fed on SL 688 (14.40 %) While the lowest intensity of gut trypsin inhibition activity was observed in genotypes NRC 94 (0.12%). A highly significant and negative correlation was observed between MLAC (cm2) by S. litura and S. obliqua and protein content in 33, leaves (r= -0.728**) and (-0.674**) respectively and trypsin inhibitor in leaves (r= -0.909**) and (r= -0.913**). Thus it can be concluded that the genotypes which were having higher protein and trypsin inhibitor in their leaves offered resistance against S. litura and S. boliqua in soybean.


Keywords: Spodopetra litura and Spilosoma obliqua larvae.

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How to cite this article:

Anchala Nautiyal, Neeta Gaur and Kamendra Singh. 2017. Effect of Soybean Leaf Protease Inhibitor on the Mean Leaf Area Consumed by Spodopetra litura and Spilosoma obliqua larvae.Int.J.Curr.Microbiol.App.Sci. 6(11): 1435-1444. doi: https://doi.org/10.20546/ijcmas.2017.611.171
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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