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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(5): 926*933
DOI: http://dx.doi.org/10.20546/ijcmas.2016.505.097


Isolation and Characterization of Lipase Producing Bacteria from Windrow Compost
V.P. Shaini1 and S. Jayasree2*
1Research and Development Centre, Bharathiar University, Coimbatore, TamilNadu, India
2Department of Zoology, Mercy College, Palakkad, Kerala-678006, India
*Corresponding author
Abstract:

Lipolytic enzymes are currently attracting significant attention because of their biotechnological potential. Most of the lipases used in industry are microbial enzymes, of both fungal and bacterial origin. Windrow composting is a controlled, self-heating, aerobic degradation of organic materials by mesophilic and thermophilic microorganisms. The present study deals with isolation of lipase producing bacteria from windrow compost. The samples were collected from compost heap at an interval of 10 days and screened for lipase producing bacterial fauna in Tributyrin agar medium after serial dilution.  A clear zone of hydrolysis indicated enzyme production. Out of 73 bacterial colonies screened, 24 lipase producing bacterial strains were isolated by checking the production of zone clearance in the tributyrin agar medium. Based on the morphological and biochemical characteristics 24 bacterial strains were identified up to species levels with the help of ABIS online analysis tool. The best lipase producers Staphylococcus (WCS1C2) and Pseudomonas (WCS3C2) genus were further characterized by 16s rDNA sequencing and identified as Staphylococcus saprophytics and Pseudomonas otidis.  These bacterial strains can be used for the production of lipase enzyme for industrial applications.


Keywords: Windrow compost,Lipase, Staphylococcus, Pseudomonas, ABIS online.
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How to cite this article:

Shaini, V.P.,  and Jayasree, S. 2016. Isolation and Characterization of Lipase Producing Bacteria from Windrow Compost.Int.J.Curr.Microbiol.App.Sci. 5(5): 926*933. doi: http://dx.doi.org/10.20546/ijcmas.2016.505.097