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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 1055-1058
DOI: https://doi.org/10.20546/ijcmas.2017.611.123


Role of Trichostatin A as Reprogramming Enhancer on In Vitro Development of Cloned Embryos: A Review
Sajad A. Beigh*, Wani A. Ahad, Rumase A. Bhat, Neelofar Nabi, Touqeer Ahmed, Mehrajuddin Reshi and Riaz A. Shah
Faculty of Veterinary Science and Animal Husbandry, Sher-e-Kashmir University of Agricultural Science and Technology of Kashmir, Shuhama, Alusteng, India
*Corresponding author
Abstract:

In cloning during reconstruction of embryo, the oocyte cytoplast reprograms the DNA of a donor nucleus, changing its status from a somatic state to an embryonic state. This remarkable change is due to the unique combination of reprogramming factors (DNA-associating factors) present in the oocyte. The reprogramming factors in the oocyte are not suitably designed to handle the epigenetic status of the somatic cells which often results in an incomplete transition due to inadequate remodelling of the donor nucleus. Recently various reprogramming enhancers have been used either on the developing cloned embryos or on donor cells which check the epigenetic errors by altering histone acetylation levels and increase the developmental competence of cloned embryos. Trichostatin A (TSA), a histone deacetylase inhibitor (HDACi) holding promise for increasing the developmental competence of cloned embryos produced either by somatic cell nuclear transfer (SCNT) or by hand-made cloning (HMC) technique in different species.


Keywords: HDACi, TSA, SCNT, HMC.
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How to cite this article:

Sajad A. Beigh, Wani A. Ahad, Rumase A. Bhat, Neelofar Nabi, Touqeer Ahmed, Mehrajuddin Reshi and Riaz A. Shah. 2017. Role of Trichostatin A as Reprogramming Enhancer on In Vitro Development of Cloned Embryos: A Review.Int.J.Curr.Microbiol.App.Sci. 6(11): 1055-1058. doi: https://doi.org/10.20546/ijcmas.2017.611.123