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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(5): 902-909
DOI: http://dx.doi.org/10.20546/ijcmas.2016.505.093


Isolation, Characterization and Optimization of Lipase Producing Pseudomonas spp. from Oil Contaminated Sites
B.N. Shukla1* and P.V. Desai2
1Department of Microbiology, Arts, Science and Commerce College- Kholwad; Kamrej Char Rasta, Surat (Gujarat), India
2Department of Biosciences, VNSG University, Surat (Gujarat), India
*Corresponding author
Abstract:

This study aims to isolate different bacteria characterize and screen them for the production of lipase. Total eight different bacterial isolates showing good extracellular lipase activity were isolated on tributyrin agar plates from oil contaminated sites. Out of which BN-2 showed moderate lipase activityin the production medium (basal medium) using olive oil as substrate. Lipase activity was quantified spectrophotometrically at 410 nm. Effect of different pH, temperature and various carbon sources was optimized and lipase activity was assayed.


Keywords: Tributyrine agar plate,Lipase activity.
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How to cite this article:

Shukla, B.N., and Desai, P.V. 2016. Isolation, Characterization and Optimization of Lipase Producing Pseudomonas spp. from Oil Contaminated Sites.Int.J.Curr.Microbiol.App.Sci. 5(5): 902-909. doi: http://dx.doi.org/10.20546/ijcmas.2016.505.093