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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(5): 878-884
DOI: http://dx.doi.org/10.20546/ijcmas.2016.505.091


Identifying the Strains having Ability to Produce Ligninolytic
Enzyme Laccase from the Plethora of Actinomycetes Isolated
from Soil and Mangrove Samples
Lalita Limaye* and R.C. Patil
Microbiology Department, Bhavan’s college, Andheri (W), Mumbai- 58, India
*Corresponding author
Abstract:

To identify laccase producing actinomycete strains a different approach was used in this study. Actinomycete strains were first grown in the presence of an inducer to better the enzyme induction. Later these strains were screened for the production of laccase enzyme which is very scarce in this group of bacteria. 62 actinomycete strains were isolated from the soil and mangrove samples collected from various locales. Aromatic inducers like guaiacol, 2,5 xylidine and tween 80 at the concentrations of 1mM were incorporated in the liquid medium. Also a lignin rich natural inducer pearl millet (bajra) powder at the concentration of 8% was incorporated in the liquid medium. The induction process with the variety of inducers was carried out for all the isolates for 2 weeks. After induction, enzyme activity was detected qualitatively by incorporating guaiacol in the medium. One isolate showed a reddish brown halo around the colony after 72 hours of incubation at 370C indicating laccase positive activity. Laccase activity of this isolate was further confirmed by decolorization of two dyes RBBR and congo red.


Keywords: Laccase, actinomycetes, inducer,RBBR,congo red.
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How to cite this article:

Lalita Limaye and R.C. Patil. 2016. Identifying the Strains having Ability to Produce Ligninolytic Enzyme Laccase from the Plethora of Actinomycetes Isolated from Soil and Mangrove SamplesInt.J.Curr.Microbiol.App.Sci. 5(5): 878-884. doi: http://dx.doi.org/10.20546/ijcmas.2016.505.091