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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 4931-4942
DOI: https://doi.org/10.20546/ijcmas.2017.610.466


A Protocol for Callus Induction in Chilli Genotypes from Hypocotyls as Explant
Sanjeev Kumar1, Naresh Mehta2, Jaywant Kumar Singh2, Manoj Kumar2* and Anil Kumar2
1Indian Institute of Soybean research, Indore, India
2Department of Plant Pathology, College of Agriculture, CCS HAU, Hisar 125 004, India
*Corresponding author
Abstract:

The yield potential as well as quality of chilli is affected by a number of biotic and abiotic stresses. The genetic improvement of this crop, especially for characters like resistance to biotic and abiotic stresses using conventional breeding methods are laborious, time taking and also needs a large experimental area. So, an attempt was made to study callus induction in chilli genotypes by using hypocotyls as explant to complement conventional breeding and expedite capsicum improvement. The study was carried out on callogenic response of chilli species with different hormones revealed that MS medium supplemented with NAA 2.5 mg/1+ BAP 2.5 mg/1 was found most suitable for callus induction to both the genotypes viz., Capsicum annuum and Capsicum frutescens. When NAA and BAP were used in combination early initiation of the callus was observed with fragile and pale yellow colour in both the genotypes of chilli. Among the chilli genotypes evaluated, the average callus formation was (77.24%) in case of C. annuum, whereas (79.20%) in C. frutescens. Hence, callus induction was significantly better in C. frutescens as compared to C. annuum.


Keywords: Callus, Chilli, Growth regulators, Tissue culture, Hypocotyls.
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How to cite this article:

Sanjeev Kumar, Naresh Mehta, Jaywant Kumar Singh, Manoj Kumar and Anil Kumar. 2017. A Protocol for Callus Induction in Chilli Genotypes from Hypocotyls as Explant.Int.J.Curr.Microbiol.App.Sci. 6(10): 4931-4942. doi: https://doi.org/10.20546/ijcmas.2017.610.466