International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:10, October, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 4901-4905

In-vitro Regeneration of Bambusa balcooa (Bamboo) through Nodal Segments
Imran Ansari, Saurabh Gupta*, Shilpi Soni and Aasif Baig
Shri Mukund Biotech, Jabalpur (M.P.), India
*Corresponding author

Bambusa balcooa (a commercial bamboo) are the most important and fastest growing species. Bambusa balcooa micro propagation protocol was established for a consistent supply of quality plants. The surface sterilization of nodal explants using 0.1% mercuric chloride followed by initiation in solid Murashige and Skoog (MS) media supplemented with BA 0.5-1.5 mg/L as growth regulator. Shoots were multiplied using MS medium with different concentrations of BA 0.5-1.5 mg/L. In the present study we are reported that when we gradually increases BA concentration from 0.5-1.5 mg/L the number of shoots were also increases and maximum shoots were reported in BA concentration 1.5 mg/L. Then 3-4 shoots were transferred for rooting media with NAA 0.5-1.5mg/L for 2-3 weeks. During the rooting we noticed that when we increases NAA concentrations from 0.5-1.5 mg/L there are gradually increase in number of roots per plantlets and the maximum rooting were observed in NAA concentration 1.5 mg/L. After root development hardening were done using a mixture of coco-peat and vermi-compost.

Keywords: Bambusa balcooa, in-vitro, MS media, Mass multiplication

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How to cite this article:

Imran Ansari, Saurabh Gupta, Shilpi Soni and Aasif Baig. 2017. In-vitro Regeneration of Bambusa balcooa (Bamboo) through Nodal Segments.Int.J.Curr.Microbiol.App.Sci. 6(10): 4901-4905. doi:
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