International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:10, October, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 4894-4900

Interspecific Crossing Barriers in Sesame (Sesamum indicum L.)
Vikas V. Kulkarni*, C.N. Ranganatha and I. Shankergoud
AICRP on Sunflower, Main Agri. Research Sciences, UAS-Raichur-584104, Karnataka, India
*Corresponding author

The genus sesamum has 38 species along with cultivated species S. indicum. Some of the wild species are known to have useful genes like disease and pest resistance. The interspecific hybridization utilizing cultivated and wild species of sesamum can lead to broadening both nuclear and cytoplasmic genetic base of the cultivated species. It has been difficult to produce interspecific hybrids due to incongruity fertilization barriers. The barriers to hybridization can occur at any stage from pollination to fertilization or even at later stages of development of the hybrid plants. The information on type of barriers which exists between cultivated and wild species is prerequisite to develop methods to overcome fertilization barriers and further to develop successful hybrids in any wide hybridization programmes. The crossability barriers between S. malabaricum and S. mulayanum with cultivated species (S.indicum) were studied using Aniline blue fluorescent microscopy technique. Pistils of different interspecific crosses were fixed to study pollen germination, pollen tube entry in papillae, style and micropylar region at different intervals after pollination. The fixed pistils of cross between S. malabaricum with cultivated species recorded 68.5% of pollen germination as compared to 90% in selfed pistils whereas S.mulayanum recorded 62.5% pollen germination as against 91% in selfed pistils. The ovules showed an average of 60% and 58% of micropylar entry as compared to 83% and 79% in selfed pistils of S. malabaricum and S. mulayanum, respectively. These results of fluorescent microscopy study indicated nonexistence of pre-fertilization barriers between wild (S. mulayanum and S. malabaricum) with cultivated species of sesame.

Keywords: Sesame, Wild species, Fertilization barriers, Fluorescent microscopy.

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How to cite this article:

Vikas V. Kulkarni, C.N. Ranganatha and Shankergoud, I. 2017. Interspecific Crossing Barriers in Sesame (Sesamum indicum L.).Int.J.Curr.Microbiol.App.Sci. 6(10): 4894-4900. doi:
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