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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 4793-4797
DOI: https://doi.org/10.20546/ijcmas.2017.610.446


Seroprevalence Study of Brucella Infection in and around Rewa District, India
Vandana Gupta1*, Namrata Singh2, Sanjay Kumar Shukla2, Varsha Sharma1, Anju Nayak1, Joycee Jogi1, Poonam Shakya1 and Ajay Rai1
1Department of Veterinary Microbiology, College of Veterinary Science and A.H., Jabalpur, M.P., India
2Department of Veterinary Microbiology, College of Veterinary Science and  A.H., Anand, Gujarat, India
*Corresponding author
Abstract:

The present work was conducted to study the seroprevalence of Brucella infection amongst the cattle population in and around Rewa district (Madhya Pradesh). Seroprevalence study was conducted using Rose Bengal Plate Test and SVANOVIR Brucella Antibody c-ELISA kit. C-ELISA is OIE referred gold standard test for detecting Brucella antibodies. Around 50 serum samples were screened for the presence of Brucella antibody using both Rose bengal plate test (RBPT), and Competitive Enzyme Linked Immunosorbent assay (c- ELISA) kit. Out of 50 samples tested with Rose Bengal Plate Test and c-ELISA, 42% and 20% were detected to be positive respectively. Rose Bengal Plate Test was not sensitive enough to detect weak positive sample, which were detected by c-ELISA, considering it as gold standard test for seroprevalence study of Brucella infection.


Keywords: Antibrucella antibodies, Brucellosis, c- ELISA, RBPT, Seroprevalence
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How to cite this article:

Vandana Gupta, Namrata Singh, Sanjay Kumar Shukla, Varsha Sharma, Anju Nayak, Joycee Jogi, Poonam Shakya and Ajay Rai. 2017. Seroprevalence Study of Brucella Infection in and around Rewa District.Int.J.Curr.Microbiol.App.Sci. 6(10): 4793-4797. doi: https://doi.org/10.20546/ijcmas.2017.610.446