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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 3654-3663
DOI: https://doi.org/10.20546/ijcmas.2017.610.429


Methicillin Resistance and its Clinical Correlation at a Tertiary Care Centre
Ganapuram J. Archana1*, Kammili Nagamani2 and Akhauri Y. Sinha3
1Department of Microbiology, Kamineni Institute of Medical Sciences, Narketpally, India
2Department of Microbiology, Gandhi Medical College, Secunderabad, India
3Ella Foundation, Genome Valley, Turkapally, Shameerpet Mandal, Hyderabad, India
*Corresponding author
Abstract:

Methicillin-Resistant Staphylococcus aureus (MRSA) is responsible for an increasing number of serious hospital and community acquired infections. Methicillin resistance has become an important problem in the community. Thus it is very essential to study the clinical correlation to know the significance. This study reports the prevalence of MRSA isolated at a tertiary care centre, Hyderabad and the clinical correlation. A total of 110 consecutive S. aureus isolates were obtained from clinical samples during the study period of Jan 2013-May 2014. All the strains were tested phenotypically by conventional methods and genotypically by Uniplex-PCR targeting S. aureus nuc gene, Panton-valentine leucocidin (PVL) toxin gene and mec A reisistance genes. Of these 82 (74.5%) of the total number of the isolates were recovered from pus/ exudates (skin and wound infections) followed by blood 21 (19%) and sputum (lower respiratory tract infection) 7 (6.3%). 53(48%) isolates of MRSA caused infections. All the 110 strains previously identified phenotypically as S.aureus with bacteriological examination were positive for amplification of 460 base pair fragments specific for nuc gene of S.aureus. Moreover, 48% of S. aureus were positive for amplification of 300 base pair fragments specific for mec A gene, while only 47% of MRSA were positive for amplification of 976 base pair fragments specific for PVL gene. Methicillin resistance was 48 %


Keywords: MRSA, PVL gene, Staphylococcus aureus, Uniplex PCR.
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How to cite this article:

Ganapuram J. Archana, Kammili Nagamani and Akhauri Y. Sinha. 2017. Methicillin Resistance and its Clinical Correlation at a Tertiary Care Centre.Int.J.Curr.Microbiol.App.Sci. 6(10): 3654-3663. doi: https://doi.org/10.20546/ijcmas.2017.610.429