International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:10, October, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 3608-3615

Supercritical Fluid Extraction of Oil from Sweet Flag Rhizome (Acorus calamus L.) and Its Insecticidal Activity on Pulse Beetles (Callosobruchus maculatus)
Shreelaxmi1*, H. Sharanagouda1, C.T. Ramachandra1, R.S. Roopa1 and S.G. Hanchinal2
1Department of Processing and Food Engineering, College of Agricultural Engineering, University of Agricultural Sciences, Raichur-584102, Karnataka, India
2Department of Entomology, College of Agriculture, University of Agricultural Sciences, Raichur- 584 102, Karnataka, India
*Corresponding author

Sweet flag rhizome (Acorus calamus L.) oil was extracted by supercritical fluid extraction method. Different temperature levels (45, 55 and 65 °C) and pressure levels (100, 150 and 200 bar) were selected for the optimization of the parameters for SC-CO2 extraction process. The highest extraction yield of 3.15 g/100 g at the rate of 90.08% were obtained at 45 °C and pressure of 200 bar. The quality of sweet flag rhizome oil was analysed and determined by using HPLC and the β-asarone content in oil were recorded at the rate of 26.80%. Further, the oil has been tested for its insecticidal activity against pulse beetles with different levels of concentration (10 to 70 μl/ml). The insecticidal activity of oil was found to be increased with increase in oil concentration and exposure time. The highest mortality of pulse beetles were observed in treatment T7 (70 μl/ml) on 7th day of storage.

Keywords: Supercritical fluid, β-asarone, sweet flag rhizome, insecticides, SC-CO2

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How to cite this article:

Shreelaxmi, H. Sharanagouda, C.T. Ramachandra, R.S. Roopa and Hanchinal, S.G. 2017. Supercritical Fluid Extraction of Oil from Sweet Flag Rhizome (Acorus calamus L.) and Its Insecticidal Activity on Pulse Beetles (Callosobruchus maculatus).Int.J.Curr.Microbiol.App.Sci. 6(10): 3608-3615. doi:
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