National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The current study reports the occurrence and molecular toxinotyping of Clostridium perfringens (C. perfringens) isolated from sheep and goat in Kashmir valley. A total of 462 faecal samples were collected from different organized and unorganized sheep and goat farms of the Kashmir valley. The samples consisted of 223 samples from sheep (adult-138, lamb-46) and goats (adult-15, kid-24) without diarrhoea (healthy) and 239 samples from sheep (adult-71, lamb-121) and goats (adult-17, kid-13) with diarrhoea. C. pefringens was isolated from 286 samples using Robertson’s cooked meat media and Sulphite Polymixin Sulphadiazine (SPS) agar. The isolates were confirmed by 16S rRNA specific PCR. Out of the 138 adult sheep and 46 lamb samples without diarrhoea, C. perfringens was obtained from 63(45.6%) and 27(58.6%) samples, respectively. Similarly, 51(71.8%) and 91(76%) samples from 71 adult sheep and 121 lambs with diarrhoea, respectively yielded C. perfringens. Likewise, 8(53.3%) and 16(66.6%) isolates were recovered from 15 and 24 healthy adult goat and kid samples, respectively. The diarrhoeic samples yielded 17(58.6%) and 13(72.2%) isolates from adult goats and kids, respectively. Among the 286 isolates from 462 samples, 228(79.72%) were typed as toxinotype A, 197(86.4%) of which had only cpa gene and 31(13.6%) carried both cpa and cpe gene. Other 58(20.2%) isolates harboured both cpa and etx genes and thus were designated as toxinotype D. Majority of the type D isolates (58.6%) were from diarrhoeic lamb and this is followed by healthy lamb (15.5%), diarrhoeic adult sheep (8.6%) and others (3.5-5.2%). None of the isolates belonged to C. perfringens type B, C or E as they were negative for cpb or cpi genes.
Citations |
 |
 |
 |
 |
