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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 3174-3184
DOI: https://doi.org/10.20546/ijcmas.2017.610.372


Molecular Typing of Clostridium perfringens Isolates from Faecal Samples of Healthy and Diarrhoeic Sheep and Goats in Kashmir
S. Rasool1, I. Hussain1, S.A. Wani1, Z.A. Kashoo1, Q. Beigh1, Q. Nyrah1, N. Nazir1, T. Hussain2, A.H. Wani1 and S. Qureshi1
1Bacteriology Laboratory, Division of Veterinary Microbiology and Immunology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir,Shuhama (Alusteng), Srinagar 190006, India
2Division of Veterinary Clinical Medicine, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shuhama (Alusteng), Srinagar 190006, India
*Corresponding author
Abstract:

The current study reports the occurrence and molecular toxinotyping of Clostridium perfringens (C. perfringens) isolated from sheep and goat in Kashmir valley. A total of 462 faecal samples were collected from different organized and unorganized sheep and goat farms of the Kashmir valley. The samples consisted of 223 samples from sheep (adult-138, lamb-46) and goats (adult-15, kid-24) without diarrhoea (healthy) and 239 samples from sheep (adult-71, lamb-121) and goats (adult-17, kid-13) with diarrhoea. C. pefringens was isolated from 286 samples using Robertson’s cooked meat media and Sulphite Polymixin Sulphadiazine (SPS) agar. The isolates were confirmed by 16S rRNA specific PCR. Out of the 138 adult sheep and 46 lamb samples without diarrhoea, C. perfringens was obtained from 63(45.6%) and 27(58.6%) samples, respectively. Similarly, 51(71.8%) and 91(76%) samples from 71 adult sheep and 121 lambs with diarrhoea, respectively yielded C. perfringens. Likewise, 8(53.3%) and 16(66.6%) isolates were recovered from 15 and 24 healthy adult goat and kid samples, respectively. The diarrhoeic samples yielded 17(58.6%) and 13(72.2%) isolates from adult goats and kids, respectively. Among the 286 isolates from 462 samples, 228(79.72%) were typed as toxinotype A, 197(86.4%) of which had only cpa gene and 31(13.6%) carried both cpa and cpe gene. Other 58(20.2%) isolates harboured both cpa and etx genes and thus were designated as toxinotype D. Majority of the type D isolates (58.6%) were from diarrhoeic lamb and this is followed by healthy lamb (15.5%), diarrhoeic adult sheep (8.6%) and others (3.5-5.2%). None of the isolates belonged to C. perfringens type B, C or E as they were negative for cpb or cpi genes.


Keywords: Clostridium perfringens, Sheep, Goat, Toxinotype, Multiplex PCR.
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How to cite this article:

Rasool, S., I. Hussain, S.A. Wani, Z.A. Kashoo, Q. Beigh, Q. Nyrah, N. Nazir, T. Hussain, A.H. Wani and Qureshi, S. 2017. Molecular Typing of Clostridium perfringens Isolates from Faecal Samples of Healthy and Diarrhoeic Sheep and Goats in Kashmir.Int.J.Curr.Microbiol.App.Sci. 6(10): 3174-3184. doi: https://doi.org/10.20546/ijcmas.2017.610.372