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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 2900-2913
DOI: https://doi.org/10.20546/ijcmas.2017.610.343


High Performance Liquid Chromatography Method for the Estimation of Water Soluble Vitamin in Five Wild Edible Fruits Consumed by the Tribal People of North-Eastern Region in India
Tapan Seal* and Kausik Chaudhuri
Plant Chemistry Department, Botanical Survey of India, Acharya Jagadish Chandra Bose Indian Botanic Garden, Shibpur, Howrah-711103, West Bengal, India
*Corresponding author
Abstract:

A reversed-phase high-performance liquid chromatographic method has been developed for the simultaneous quantitation of water-soluble vitamins like ascorbic acid (C), thiamine (B1), riboflavin (B2), niacin (B3), pantothenic acid (B5), pyridoxine (B6) and folic acid (B9) in five potent wild edible fruits named Morus indica, Meynalaxi flora, Solanum gilo, Solanum kurzii and Viburnum foetidum, consumed by the tribal people of North-eastern region in India. The experimental results showed that for different fruits, the vitamin C content ranged between 6.23 ± 0.04 and 51.59 ±0.40 mg/100g dry plant materials. The B1 content was determined high in M. laxiflora (1.10± 0.010 mg/100g and minimum in the fruits of S. kurzii. A significant amount of B3 (15.04 ± 0.08 mg/100gm) and good amount of B2 (1.44 ±0.006 mg/100gm) and B9 (0.95 ±0.0 mg/100gm) were detected in M. laxiflora. The results of investigation showed that these fruits are rich sources of vitamins that can contribute immensely to nutrition and food security. The high percentage of recovery and low limit of detection confirm the suitability of the method for simultaneous quantification of vitamins in these five fruits.


Keywords: Wild edible plants, Water soluble vitamins, B group vitamins, Vitamin C, HPLC Analysis.
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How to cite this article:

Tapan Seal and Kausik Chaudhuri. 2017. High Performance Liquid Chromatography Method for the Estimation of Water Soluble Vitamin in Five Wild Edible Fruits Consumed by the Tribal People of North-Eastern Region in India.Int.J.Curr.Microbiol.App.Sci. 6(10): 2900-2913. doi: https://doi.org/10.20546/ijcmas.2017.610.343