International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:10, October, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 2713-2721

Phosphate Solubilization by Endophytic Bacteria isolated from Oryza sativa
Madhusmita Borah1, Pompi Das2, Susanta Sarmah Pathak2, Robin Chandra Boro2 and Madhumita Barooah2*
1Centre for Biotechnology and Bioinformatics, Dibrugarh University, Dibrugarh, Assam, India
2Department of Agricultural Biotechnology, Assam Agricultural University,Jorhat-13, Assam, India
*Corresponding author

Endophytic bacteria isolated from the Oryza sativa plant were evaluated for phosphate solubilisation. Five of these isolates that showed good phosphate solubilising activity were characterised at molecular level and tested for phosphate solubilisation using three different sources viz tricalcium phosphate (TCP), aluminium phosphate (AlPO4) and iron phosphate (FePO4). Based on 16s rRNA gene sequence analysis the isolates were identified as Pantoeaa ananatis, Pseudomonas putida, Brevibacillus agri, Bacillus subtilis and Bacillus megaterium. The isolates showed maximum phosphate solubilizing activity on the 6th day of incubation (DAI) with a concomitant decrease in the pH of the medium. Bacillus subtilis (LP31 L03) showed highest phosphate solubilising activity (57.58±0.65, 6.10±0.65, 7.65±0.30 μg/ml) in TCP, AlPO4 and FePO4 respectively. The isolates under study were able to easily solubilize TCP followed by FePO4. Among the sources tested, AlPO4 was least solubilised. The decrease in pH of the medium inoculated with Bacillus subtilis (LP31 L03) was recorded as 4.02±0.12, 3.33±0.19 and 3.8±0.14 in TCP, AlPO4 and FePO4 respectively on 6th DAI. The study reports that endophytic isolates have good phosphate solubilizing activity and indicates and can be good candidate strains for developing an effective phosphate solubilizing biofertilizer.

Keywords: Endophytes, Phosphate solubilization, Biofertilizer, Tricalcium phosphate, Aluminium phosphate, Ferric phosphate, DAI (Days after Inoculation).

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How to cite this article:

Madhusmita Borah, Pompi Das, Susanta Sarmah Pathak, Robin Chandra Boro and Madhumita Barooah. 2017. Phosphate Solubilization by Endophytic Bacteria isolated from Oryza sativa.Int.J.Curr.Microbiol.App.Sci. 6(10): 2713-2721. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.