International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:10, October, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 2532-2542

In Vitro Evaluation of Bio-Control Agents, Biopesticides and Botanicals against Colletotrichum truncatum Causing Anthracnose of Horse Gram (Macrotyloma uniflorum)
Devanshi Pandit* and R.P. Kaushal
Dept. of Plant Pathology CSK HP Agricultural University, Palampur-176062, H.P., India
*Corresponding author

The experiment was conducted under in vitro to observe the effect of bio-agents, biopesticides and botanicals against Colletotrichum truncatum. Antagonistic activity of bioagents viz., Trichoderma viride-H (Hydrabad strain), T. harzianum-H (Hydrabad strain) and Pseudomonas fluorescens (TNAU strain) against C. truncatum was tested using dual-culture technique. Maximum inhibition of mycelial growth was observed with T. viride-H (58.81%) followed by P. fluorescens-TNAU (46.03%) and T. harzianum-H (35.47%). In vitro evaluation of neem based biopesticides was carried out by poisoned food technique. For this three neem based biopesticides viz., Neemban, Neembenicide and Achook were tested at 0.05, 0.1 and 0.5 per cent concentrations for inhibitory activity on mycelial growth. Achook was found most effective and resulted in maximum inhibition of 52.34, 57.03 and 66.40 per cent followed by Neembenicide which showed 29.66, 45.29 and 59.37 per cent inhibition at 0.05, 0.1 and 0.5 per cent concentrations, respectively. Eight locally available plants which grow in abundance viz., basuti (Adhatoda vasica), bhang (Cannabis sativa), kali basuti (Chromolaena odorata), barein (Acorus calamus), butter-cup (Ranunculus bulbosus), safeda (Eucalyptus globulus), curry plant (Murraya koenigii) and pine needles (Pinus longifolia) were used and aqueous extracts of different concentrations (20, 40, 60, 80 and 100%) were tested for their efficacy against the pathogen. An increased inhibitory effect of mycelial growth was observed with an increase in concentration from 20 to 100 per cent. Cannabis sativa at 100 per cent was found best (81.23%) followed by Chromolaena odorata (80.46%) and Adhatoda vasica (67.96%). At 20 per cent Eucalyptus globulus was found to be most effective (35.14%) followed by Pinus longifolia (19.51%) and Acorus calamus (17.14%). At 40 (49.24%), 60 (60.13%) and 80 (70.28%) per cent concentration Chromolaena odorata was found to be effective.

Keywords: Bio-control agents, Botanicals, Biopesticides, Colletotrichum truncatum, Horse Gram.

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How to cite this article:

Devanshi Pandit and Kaushal, R.P. 2017. In Vitro Evaluation of Bio-Control Agents, Biopesticides and Botanicals against Colletotrichum truncatum Causing Anthracnose of Horse Gram (Macrotyloma uniflorum).Int.J.Curr.Microbiol.App.Sci. 6(10): 2532-2542. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.