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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 2493-2502
DOI: https://doi.org/10.20546/ijcmas.2017.610.293


Genotypic Characterization of Indigenous Rhizobium Strain from Cultivated Cowpea (Vigna unguiculata L.) in Bangladesh
Ali Mohammad Nushair, Ananda Kumar Saha, Md. Anisur Rahman, Moni Krishno Mohanta* and Md. Fazlul Haque
Genetics and Molecular Biology Laboratory, Department of Zoology, University of Rajshahi, Rajshahi-6205, Bangladesh
*Corresponding author
Abstract:

A gram negative strain of Rhizobium sp. SOY7 has been isolated from root nodules of cow pea (Vigna unguiculata L.) which was later identified on the basis of biochemical tests and 16S rDNA sequencing. The optimum culture condition of Rhizobium sp. SOY7 was determined at pH 7.0 and 28ËšC temperature. The strain was resistant to most of the antibiotics used in this study except Tetracycline, Ceftazidime, Pefloxacin, Cotrimoxazole and Mecillinam. Moreover, the isolate was sensitive to the higher concentration of NaCl (>1%). The strain is capable to produce nodules when induced rhizobia as inoculants and resulted insignificant changes in plant growth characteristics. The most abundant nodulation and improved growth of the legumes were determined in plants inoculated with Rhizobium sp. SOY7 was statistically significant (p < 0.05) compared with uninoculated controls.


Keywords: Rhizobium, Cowpea, 16S rDNA sequence, Nodulation.
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How to cite this article:

Ali Mohammad Nushair, Ananda Kumar Saha, Md. Anisur Rahman, Moni Krishno Mohanta and Md. Fazlul Haque. 2017. Genotypic Characterization of Indigenous Rhizobium Strain from Cultivated Cowpea (Vigna unguiculata L.) in Bangladesh.Int.J.Curr.Microbiol.App.Sci. 6(10): 2493-2502. doi: https://doi.org/10.20546/ijcmas.2017.610.293