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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 2474-2492
DOI: https://doi.org/10.20546/ijcmas.2017.610.292


Molecular Characterization of Cellulolytic Bacteria Derived From Termite Gut and Optimization of Cellulase Production
V.S. Shinde1*, T. Agrawal1 and A.S. Kotasthane2
1Department of Plant Molecular Biology and Biotechnology, IGKV, Raipur-492012, India
2Department of Plant Pathology, IGKV, Raipur-492012, India
*Corresponding author
Abstract:

Cellulolytic bacteria were previously isolated from the termite guts and efforts were taken to optimize parameters for cellulase production. Activities of diversity of enzymes which include, CMCase, avicelase and filterpaperase (FPase) were studied from crude cellulase extracts. CMCase activity was found optimum at different temperatures (35°C, 40°C and 45°C) at pH 7 and after 48 hrs of incubation time. Avicelase and FPase activities were found optimum at 35°C, pH 7 and after 48 hours of incubation. Results clearly indicated that there is a great impact of temperature, pH and incubation period on cellulase production. Isolate C3 and Z were found highest CMCase and FPase producers respectively. 16s rRNA gene analysis confirmed that, isolates belong to different species of Pseudomonas, Klebsiella, Salmonella, Serratia and Enterobacter. Molecular characterization using REP, ERIC and BOX sequences showed high-moderate level of diversity among these isolates. Growth profiles of isolates showed that, they can grow best between temperatures 30°C-40°C at pH 7.


Keywords: Cellulase, DNS, Optimization, 16s rRNA identification, ERIC-REP-BOX.
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How to cite this article:

Shinde, V.S., T. Agrawal and Kotasthane, A.S. 2017. Molecular Characterization of Cellulolytic Bacteria Derived From Termite Gut and Optimization of Cellulase Production.Int.J.Curr.Microbiol.App.Sci. 6(10): 2474-2492. doi: https://doi.org/10.20546/ijcmas.2017.610.292