International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:10, October, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 2295-2303

Prevalence of ESBL Producing Escherichia coli and Klebsiella Species among Clinical Isolates and their in vitro Antimicrobial Susceptibility Pattern in a Tertiary Care Hospital
Sanjo Gupta, Veena Maheshwari* and Rajesh Shah
Department of Microbiology, RKDFMCH & RC, Bhopal, India
*Corresponding author

E. coli and Klebsiella spp. are the main opportunistic pathogens under family Enterobacteriaceae. Cephalosporins are the first line of drugs against these organisms, but the inappropriate use of beta-lactam antibiotics render these organisms more vulnerable for the production of enzymes. Prevalence of ESBLs has resulted in widespread development of drug resistance. The present study was done to detect the prevalence of ESBLs producing E. coli and Klebsiella spp with their antibiotic resistance patterns at TMMC Tertiary care hospital. A total no of 200 isolates were obtained from clinical samples from March to February 2015. Samples were inoculated on MacConkey and sheep blood agar. The organisms were identified based on standard microbiological techniques. Antimicrobial susceptibility testing was performed by Kirby-Bauer disc diffusion method, as per CLSI guidelines. Following screening with Cefotaxime (30 µg) disc, resistance was then confirmed for ESBL production by phenotypic confirmatory disc diffusion test using Ceftazidime (30 µg) and Ceftazidime + clavulanic acid (30 µg) disc. Result: Out of 120 Escherichia coli isolates 80 isolates of Klebsiella species, 31(25.83%) and 19 (23.75%) were ESBL producers respectively. These ESBL producers showed high-degree of resistance to ampicillin (100%) followed by ceftazidime, pipracillin and cefotaxime for both E. coli and Klebsiella species. The most sensitive antibiotics were found to be imipenem followed by Amikacin and meropenem. The present study highlights a relatively higher prevalence ESBL among E. coli and Klebsiella spp In view of these findings, we recommend that ESBL testing should be done routinely to reduce resistance to antibiotics and to strengthen the enforcement of infection control measures. It is pertinent for the treating clinicians so that they can make an informed choice as far as appropriate antibiotics and precautions are concerned.

Keywords: ESBL, E.coli, Klebsiella spp., Ceftazidime, Ceftazidime+ clavulanic acid, Multidrug resistance.

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Sanjo Gupta, Veena Maheshwari and Rajesh Shah. 2017. Prevalence of ESBL Producing Escherichia coli and Klebsiella species among clinical isolates and their invitro antimicrobial susceptibility pattern in a tertiary care hospital.Int.J.Curr.Microbiol.App.Sci. 6(10): 2295-2303. doi:
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