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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 2125-2138
DOI: https://doi.org/10.20546/ijcmas.2017.610.252


Studies on the Effect of Ascophyllum nodosum Extract (ANE) on Growth, Antioxidant Potential and Alpha Glucosidase Inhibition Activity of Vigna aconitifolia (RMO 225)
Nidhi Verma1, Krishan D. Sehrawat2 and Anita R. Sehrawat1*
1Department of Botany, Maharshi Dayanand University, Rohtak-124001, Haryana, India
2Department of Genetics and Plant Breeding, CCS Haryana Agricultural University,  Hisar, Haryana, India
*Corresponding author
Abstract:

Vigna aconitifolia is highly resistant food crop with highly nutritious seed proteins. Among different seaweeds the Ascophyllum nodosum is more researched these days, so it is important to study the interaction of the seaweed with different food crops including legumes. We previously found increased tyrosinase inhibition activity in Vigna aconitifolia seeds when treated with Ascophyllum nodosum. So this study investigated the effects of Biovita, a Seaweed Liquid Fertilizer (SLF) of Ascophyllum nodosum, on seed germination, shoot length, root length, fresh weight and dry weight of moth bean seeds. The free radical scavenging by 1, 1-diphenyl-2picrylhydrazyl (DPPH) and alpha glucosidase inhibition activity of treated seeds up to 24 hour (0, 6, 12, 18 and 24 hour) also determined. The seeds of Vigna aconitifolia were treated with 0%, 0.01%, 0.05%, 0.10%, 0.50% and 1.0% of A. nodosum extract for different time periods ranging from 0 to 24 hours. The seed germination, shoot length, root length, fresh weight and dry weight were found maximum at 0.01% of SLF. The result showed that among different concentration of SLF the 0.05 % was best for free radical scavenging and alpha glucosidase inhibition activity. From the experiment it was observed that with increased time period (0 to 24 hour) free radical scavenging and alpha glucosidase inhibition activity increased but among different concentrations of SLF the activities increased up to 0.05% of the seaweed and decrease thereafter due to toxicity in Vigna aconitifolia seeds. The study also showed a positive correlation between radical scavenging and alpha glucosidase inhibition. So seed treatment up to 0.05% was best for seed germination, shoot length, root length, fresh weight, dry weight, free radical scavenging and alpha glucosidase inhibition activity.


Keywords: Vigna aconitifolia, Ascophyllum nodosum Extract (ANE), 2, 2-diphenyl-1-picrylhydrazyl (DPPH), Alpha glucosidase inhibition.
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How to cite this article:

Nidhi Verma, Krishan D. Sehrawat and Anita R. Sehrawat. 2017. Studies on the Effect of Ascophyllum nodosum Extract (ANE) on Growth, Antioxidant Potential and Alpha Glucosidase Inhibition Activity of Vigna aconitifolia (RMO 225).Int.J.Curr.Microbiol.App.Sci. 6(10): 2125-2138. doi: https://doi.org/10.20546/ijcmas.2017.610.252