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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 1862-1873
DOI: https://doi.org/10.20546/ijcmas.2017.610.225


Genetic Variability among High Protease Productive Bacillus Mutants using RAPD-PCRa
Effat A.M. Soliman1*, S.E.M Abo-Aba2, Nivien A. Abosereh1, Nariman A.H. Aly1 and Atif D.O. Aljohani2
1Microbial Genetic Department, National Research Centre, 33 El-Bohouth St.(Former El-Tahrir St.) Dokki, P.O. 12662, Giza, Egypt
2Biological Science Department, Faculty of Science, King Abdulaziz University,Jeddah, Saudi Arabia
*Corresponding author
Abstract:

Proteases enzymes are among the most bio-products required to meet some important human needs where microbial enzymes participate a big share in the world market for enzymes. In this study, we aimed to increase the productivity of protease enzyme in Staphylococcus epidermidis, Bacillus licheniformis, B. thuringiensis, B. subtilis, B. pumilus, Pseudomonas fluorescens and P. aeruginosa via UV irradiation. We aimed also to determine the genetic variation among high protease productive mutants using RAPD-PCR. All strains showed different production levels ranged between 3.2–58 U/ml. The most efficient productive strains, B. licheniformis and B. pumilus, were used to examine the changes that occur in gene expression after exposure to UV as a physical mutagen. Out of all mutants obtained, two mutants showed higher efficiency in productivity. BLM1-10 mutant gave nearly twice as much activity produced by B. licheniformis original strain while BPM2-10 gave a six and half times higher than activity produced by B. pumilus original strain. RAPD-PCR data analysis revealed a variability of fragment sizes produced in wild type strains, B. licheniformis and B. pumilus, than their UV respective mutants.


Keywords: UV irradiation, Protease, Bacillus, RAPD analysis, Genetic variability.
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How to cite this article:

Effat A.M. Soliman, S.E.M Abo-Aba, Nivien A. Abosereh, Nariman A.H. Aly and Atif D.O. Aljohani. 2017. Genetic Variability among High Protease Productive Bacillus Mutants using RAPD-PCR.Int.J.Curr.Microbiol.App.Sci. 6(10): 1862-1873. doi: https://doi.org/10.20546/ijcmas.2017.610.225