National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Proteases enzymes are among the most bio-products required to meet some important human needs where microbial enzymes participate a big share in the world market for enzymes. In this study, we aimed to increase the productivity of protease enzyme in Staphylococcus epidermidis, Bacillus licheniformis, B. thuringiensis, B. subtilis, B. pumilus, Pseudomonas fluorescens and P. aeruginosa via UV irradiation. We aimed also to determine the genetic variation among high protease productive mutants using RAPD-PCR. All strains showed different production levels ranged between 3.2–58 U/ml. The most efficient productive strains, B. licheniformis and B. pumilus, were used to examine the changes that occur in gene expression after exposure to UV as a physical mutagen. Out of all mutants obtained, two mutants showed higher efficiency in productivity. BLM1-10 mutant gave nearly twice as much activity produced by B. licheniformis original strain while BPM2-10 gave a six and half times higher than activity produced by B. pumilus original strain. RAPD-PCR data analysis revealed a variability of fragment sizes produced in wild type strains, B. licheniformis and B. pumilus, than their UV respective mutants.
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