Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(5): 512-520
DOI: http://dx.doi.org/10.20546/ijcmas.2016.505.053


Antimicrobial Activity of Leaf Extract – Fractions of Vernonia calvoana against Selected Stock Cultures in Microbiology Laboratory, Cross River University of Technology, Calabar
B.U. Ati1*, I.A. Iwara1, A.O. Bassey2, G.O Igile1, E.E. Duke2 and P.E. Ebong1
1Department of Biochemistry, Faculty of Basic Medical Sciences,
University of Calabar, Calabar, Nigeria
2Department of Medical Laboratory Science, College of Health Technology, Calabar, Nigeria
*Corresponding author
Abstract:

This study aims to determine antimicrobial activity of the leaf extract – fractions of Vernonia calvoana against selected stock cultures in Microbiology Laboratory, Cross River University of Technology, Calabar. Agar disk diffusion method was used for screening of extract – fractions against Escherichia coli, Staphyloccus aureus, Salmonella typhi, Candida albicans, Aspergillus flavus, Pseudomonas aeruginosa and Helicobacter pylori. Out of the seven, three of the microorganisms were used to determine the minimal inhibitory concentration (MIC) owing to their profound inhibition. The crude extract, 30% and 100% methanol fractions were assayed for antimicrobial activities. The crude extract exhibited the highest antimicrobial potency against S. aureus, E. coli, and P. aeruginosa with inhibition zone diameter ranged from 20 - 22mm. The inhibitory effect of 100% methanol fraction was recorded with inhibitory zone diameter ranged from 12 – 19mm; and the 30% methanol fraction slightly inhibited with zone diameter ranged from 10 – 15mm against the test microorganisms. Vernonia calvoana plant showed antimicrobial potency against the selected stock cultures and probably could be used to control infections associated with these organisms.


Keywords: Vernonia calvoana, medicinal plant, antimicrobial, microorganism, methanol
Download this article as Download

How to cite this article:

Ati, B.U., I.A. Iwara, A.O. Bassey, G.O Igile, E.E. Duke and Ebong, P.E. 2016. Antimicrobial Activity of Leaf Extract – Fractions of Vernonia calvoana against Selected Stock Cultures in Microbiology Laboratory, Cross River University of Technology, Calabar.Int.J.Curr.Microbiol.App.Sci. 5(5): 512-520. doi: http://dx.doi.org/10.20546/ijcmas.2016.505.053