International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:10, October, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 1768-1774

Characterization of Humic Substances by Functional Groups and Spectroscopic Methods
M. Eshwar1*, M. Srilatha1, K. Bhanu Rekha2 and S. Harish Kumar Sharma1
1Department of Soil Science and Agricultural chemistry, College of Agriculture, PJTSAU, Rajendranagar, Hyderabad- 500 030, Telangana, India
2Department of Agronomy, Agriculture College, Palem, Nagarkurnool
*Corresponding author

The present study was carried in the Department of Soil Science and Agricultural Chemistry, College of Agriculture, Rajendranagar. The humic and fulvic acids were extracted, isolated and purified from manures and were studied for their functional groups and spectroscopic methods. It has been found that the total acidity (9.4 me g-1), carboxyl group (6.2 me g-1) and phenolic-OH (3.2 me g-1) groups were higher in case of fulvic acid compared to humic acid (7.2, 4.2 and 3.0 me g-1 respectively). Among these functional groups, the percent contribution of carboxylic groups towards total acidity was higher than that of phenolic-OH groups in both humic fractions. The ratio of optical densities at 465 and 665 nm i.e. E4/E6 ratio was higher in case of fulvic acid (6.17) compared to humic acid (4.34). The UV spectra of humic and fulvic acid were similar in nature in spite of the differences in their composition and showed a decreasing optical density with increasing wavelength.

Keywords: Humic acid (HA), Fulvic acid (FA), Functional groups (total acidity, carboxyl group and phenolic-OH group) E4/E6 ratio and UV spectra.

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Eshwar, M., M. Srilatha, K. Bhanu Rekha and Harish Kumar Sharma, S. 2017. Characterization of Humic Substances by Functional Groups and Spectroscopic Methods.Int.J.Curr.Microbiol.App.Sci. 6(10): 1768-1774. doi:
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