National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A total of 19 bacterial endophytes were isolated from leaves of cotton (Gossypium hirsutum) and characterized for plant growth promoting traits viz., siderophores production, phosphate solubilization, IAA and ammonia production; and preliminarily screened for chitinase, gelatinase, protease and lipase. Quantitative analysis of siderophores production and phosphate solubilization indicated that six endophyteseach viz., EB11, EB4, EB3, EB5, EB8 and EB9 were able to produce siderophores ranging between 80.26 to 92.58 % and EB9, EB11, EB14, EB17, EB4 and EB2 solubilized phosphorus ranging 10.24-13.58mg/ml within 48 hrs. After 72 hrs incubation, seven isolates EB3, EB13, EB8, EB11, EB15, EB2 and EB17 produced IAA in range of 15-28 µg/ml. Within 48 hrs, the ammonia production ranged from 0.8 to 1.6 (µg/ml). Primary screening of enzymes showed that chitinase was produced by EB5 (1.9 mm) and EB3 (1.1 mm); protease by EB15, EB12, EB11, EB1 and EB4 (11.80-16.60mm); gelatinase by EB1, EB9, EB1, EB12 and EB16 (10.00 -17.00mm); and lipase by EB10, EB13, EB17, EB14, EB12 and EB11 (10.00-15.00mm). Results suggested all endophytic bacterial strains studied are potential for the one or other parameter studied thus further study will help to make consortium as biofertilizer or bioprotectant to enhance plant productivity and protection.
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