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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 1543-1549
DOI: https://doi.org/10.20546/ijcmas.2017.610.185


Detection of Multidrug Resistance and Characterization of Mutations in Mycobacterium tuberculosis Isolates in Raichur District, India
Akshay Kumar Arya1*, R. Venkatesh Naik1, K.V. Arjun2 and B.V. Peerapur1
1Department of Microbiology, RIMS, Raichur, Karnataka, India
2Microbiologist & Incharge DST Lab, Dept. of Microbiology, RIMS, Raichur, Karnataka, India
*Corresponding author
Abstract:

Tuberculosis remains as one of the leading cause of morbidity and mortality globally. Estimated cases of around 9.6 million are affected by all forms of TB worldwide, out of which 2.2 billion are affected in India. India also has the second highest burden of MDR TB after China. Information on MDR TB is sparse in Raichur District. We undertook this study to detect MDR TB among MDR suspected cases and its common mutation using Genotype MTBDR plus. All presumptive MDR sputum samples were sent to DST LAB RIMS, Raichur from various part of Raichur district. Samples in study were taking during the period from January 2017 to April 2017. Line Probe Assay was done on sputum samples to detect common mutations in rpoB gene for Rifampicin and katG gene and inhA gene for Isoniazid resistance respectively. Out of the 448 samples received from MDR suspects, 49(10.9%) were found to be MDR TB. Commonest pattern for Rifampicin resistance was missing WT8 probe along with mutation in MUT3 band (S531L) codon, and mutation of MUT1 band (S5315T) of katG gene was commonest pattern for Isoniazid resistance. The MDR-TB among presumptive MDR in Raichur district was found to be 10.9%, and the mutation pattern obtained for Rifampicin and Isoniazid resistance were similar to those reported earlier.


Keywords: MDR-TB, Drug resistance, Line probe assay, Rifampicin and Isoniazid, Drug susceptibility testing.
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How to cite this article:

Akshay Kumar Arya, R. Venkatesh Naik, K.V. Arjun and Peerapur, B.V. 2017. Detection of Multidrug Resistance and Characterization of Mutations in Mycobacterium tuberculosis Isolates in Raichur DistrictInt.J.Curr.Microbiol.App.Sci. 6(10): 1543-1549. doi: https://doi.org/10.20546/ijcmas.2017.610.185