International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:10, October, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 1537-1542

A Retrospective Study for Detection of Inducible Resistance to Clindamycin in Staphylococcus aureus Isolates Using D-Test at RIMS Teaching Hospital, Raichur, India
R. Venkatesh Naik*, Basavaraj V. Peerapur and P. Sandhya
Department of Microbiology, RIMS, Raichur, Karnataka, India
*Corresponding author

Staphylococcus aureus is recognized as causing nosocomial and community-acquired infections in every region of the world. Clindamycin is considered as useful alternate drug in penicillin-allergic patients in the treatment of skin & soft tissue infections caused by Staphylococcus aureus. Staphylococcus spp. can be resistant to erythromycin through either erm or msr A genes. Strains with erm-mediated erythromycin resistance may possess inducible Clindamycin resistance but may appear susceptible to Clindamycin by disc diffusion test. The objective of the present study was to know the prevalence of erythromycin-induced Clindamycin resistance among clinical isolates of S. aureus. A total of 160 S. aureus isolates from various clinical samples submitted in the Dept. of Microbiology, RIMS teaching hospital, Raichur were studied. Methicillin resistant S. aureus strains were identified by Cefoxitin disc diffusion method. Inducible Clindamycin resistance was detected by erythromycin and Clindamycin disc approximation test (D-zone test) as per CLSI guidelines. Among the 160 S. aureus isolates, 75 strains (46.8%) were detected as MRSA of which 32(42.6%) strains showed inducible Clindamycin resistance (D-test positive) and 12(14.1%) isolates out of 85 strains MSSA were D-test positive. 73(45.6%) isolates of S. aureus were sensitive to both erythromycin and Clindamycin. High prevalence of strains with inducible Clindamycin resistance particularly among MRSA indicates that inducible Clindamycin resistance testing (D-test) should be included as a part of routine antibiotic susceptibility. These isolates may be missed in routine antibiotic testing by disk diffusion method.

Keywords: Clindamycin resistance, MRSA, MSSA, D test.

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How to cite this article:

Venkatesh Naik R., Basavaraj V. Peerapur and Sandhya P. 2017. A Retrospective Study for Detection of Inducible Resistance to Clindamycin in Staphylococcus aureus Isolates Using D – Test at RIMS Teaching Hospital, Raichur.Int.J.Curr.Microbiol.App.Sci. 6(10): 1537-1542. doi:
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