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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 1478-1484
DOI: https://doi.org/10.20546/ijcmas.2017.610.175


Isolation, Identification and Molecular Characterization by RAPD of Red HE7B Dye Decolourizing Bacteria from Textile Effluents and Its Cultivated Soil
A.D. Patel*, H.P. Gajera, S.V. Patel and B.A. Golakiya
Food Testing Laboratory (NABL accredited), Department of Biotechnology Junagadh Agricultural University, Junagadh-362001, Gujarat, India
*Corresponding author
Abstract:

Colours give delightful pleasure to eyesight but at the same time they may act as serious pollutants when their origin is dyes and dyestuffs. The textile industry is one of the industries that generate a high volume of waste water. Strong colour of the textile waste water is the most serious problem of the textile waste effluent. Total 26 bacterial isolates were isolated from fourteen different effluents and its contaminated and cultivated soil. Dye decolourization study was carried out using all 26 isolates with one dye (Red HE7B 100ppm) for checking potentiality of isolated bacterial isolates. After 10 days of incubation with bacterial isolates, 14 isolates gave satisfactorily decolourization above 70% compared with control. The morphological, microscopic and biochemical characterization of selected 14 bacterial isolates (Ps1 to Ps14) was carried out. All 14 bacterial isolates were found either circular or round in shape except Pseu-ghat-eff-4 which is found irregular in shape. All 14 bacterial isolates identified as Pseudomonas sp. The genomic DNA was isolated from 24 h old Pseudomonas bacterial cultures. The RAPD was performed using genomic DNA of 14 Pseudomonas sp. to generate diversified map and identification of specific markers associated with decolourization activity. Total 172 fragments were generated by 17 RAPD primers across 14 bacterial isolates. Out of 170 bands, 122 bands were shared polymorphic which was found at least more than two isolates and 48 bands were unique polymorphic bands. Jaccard similarity coefficient was found in the range of 0.137 to 0.368 in clustering pattern of 14 bacterial isolates. Best bacterial isolates, Ps4 with respect to dye decolourization was distinguished by 6 unique bands amplified by OPA-07 (356bp), OPA-18 (577), OPB-18 (1150), OPD-02 (2152), OPF-15 (1079) and OPZ-15 (2694bp) makers. However, Ps10 was distinguished by 4 unique bands amplified by OPF-06(1817bp), OPF-15(1535bp), OPO-06(2208bp) and OPO-13(484bp).


Keywords: Textile effluents, Red HE7B dye, RAPD, Pseudomonas, Molecular marker.
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How to cite this article:

Patel, A.D., H.P. Gajera, S.V. Patel and Golakiya, B.A. 2017. Isolation, Identification and Molecular Characterization by RAPD of Red HE7B Dye Decolourizing Bacteria from Textile Effluents and Its Cultivated Soil.Int.J.Curr.Microbiol.App.Sci. 6(10): 1478-1484. doi: https://doi.org/10.20546/ijcmas.2017.610.175