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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 1308-1318
DOI: https://doi.org/10.20546/ijcmas.2017.610.154


In Vitro Production of Cormels in Gladiolus hybridus through Gamma Rays
Neha Dogra* and K.K. Dhatt
Department of Floriculture and Landscaping, Punjab Agricultural University, Ludhiana–141004, Punjab, India
*Corresponding author
Abstract:

The present experiment was conducted to standardize the protocol for in vitro mutagenesis of gladiolus hybridus varieties ‘Punjab Glance’ and ‘Sylvia’. The nodal buds were irradiated with different doses of gamma rays (Co60) 0, 5, 8, 10 and 12 Gy. Irradiated explants were then cultured aseptically on MS basal medium supplemented with 5 mg/l (BAP) + 2mg/l (IBA) and agar-agar to induce shoot and root proliferation. Explants treated at higher doses (10 and 12 Gy) showed deleterious effects of ionizing radiation. Morphological abnormalities in terms of stunted growth were observed at 10 and 12 Gy of gamma rays. The minimum time taken to shoot and root (14.07), (15.06) formation, days to cormel formation (60.04 days) at 8 Gy. Shoot length (4.04 cm), number of shoots (25.07), root length (4.18 cm), number of roots per (10.04). In vitro-raised mutant cormels and non-irradiated (control) plants were transferred to plastic pots one month after acclimatisation under laboratory conditions and examined for their morphological traits. These mutated cormels were multiplied on a large-scale through micropropagation and evaluated for their stability. This study developed a mutagenesis protocol that could be used to develop novel colour mutants in gladiolus.


Keywords: Gladiolus, Nodal bud, Multiple shoots, Mutation, Cormel production and Survival per cent.
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How to cite this article:

Neha Dogra and Dhatt, K.K. 2017. In Vitro Production of Cormels in Gladiolus hybridus through Gamma Rays.Int.J.Curr.Microbiol.App.Sci. 6(10): 1308-1318. doi: https://doi.org/10.20546/ijcmas.2017.610.154