International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2022 - IJCMAS--ICV 2022: 95.28 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020] For more details click here

Login as a Reviewer

See Guidelines to Authors
Current Issues
Download Publication Certificate

Original Research Articles                      Volume : 6, Issue:10, October, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 1308-1318

In Vitro Production of Cormels in Gladiolus hybridus through Gamma Rays
Neha Dogra* and K.K. Dhatt
Department of Floriculture and Landscaping, Punjab Agricultural University, Ludhiana–141004, Punjab, India
*Corresponding author

The present experiment was conducted to standardize the protocol for in vitro mutagenesis of gladiolus hybridus varieties ‘Punjab Glance’ and ‘Sylvia’. The nodal buds were irradiated with different doses of gamma rays (Co60) 0, 5, 8, 10 and 12 Gy. Irradiated explants were then cultured aseptically on MS basal medium supplemented with 5 mg/l (BAP) + 2mg/l (IBA) and agar-agar to induce shoot and root proliferation. Explants treated at higher doses (10 and 12 Gy) showed deleterious effects of ionizing radiation. Morphological abnormalities in terms of stunted growth were observed at 10 and 12 Gy of gamma rays. The minimum time taken to shoot and root (14.07), (15.06) formation, days to cormel formation (60.04 days) at 8 Gy. Shoot length (4.04 cm), number of shoots (25.07), root length (4.18 cm), number of roots per (10.04). In vitro-raised mutant cormels and non-irradiated (control) plants were transferred to plastic pots one month after acclimatisation under laboratory conditions and examined for their morphological traits. These mutated cormels were multiplied on a large-scale through micropropagation and evaluated for their stability. This study developed a mutagenesis protocol that could be used to develop novel colour mutants in gladiolus.

Keywords: Gladiolus, Nodal bud, Multiple shoots, Mutation, Cormel production and Survival per cent.

Download this article as Download

How to cite this article:

Neha Dogra and Dhatt, K.K. 2017. In Vitro Production of Cormels in Gladiolus hybridus through Gamma Rays.Int.J.Curr.Microbiol.App.Sci. 6(10): 1308-1318. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.