National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Group A rotavirus is the leading cause of severe diarrhoea in children worldwide. So, there is need to document the prevalence of local strain(s) circulating in different regions for successful implementation of vaccine program. A total of 162 stool samples were analyzed from April 2012 to March 2013in Ludhiana and Bathinda districts of Punjab by ELISA, RNA-PAGE and RT-PCR. G and P genotyping was done by RT-PCR using genotype-specific primer sets targeting VP4 and VP7 genes. RNA PAGE, RT-PCR and ELISA showed a positive prevalence rate of 6.17%, 8.02% and 9.25%respectively. RT-PCR showed perfect agreement with other two diagnostic techniques as per Diagnostic Test Agreement. In G genotyping G1 (66.66%) showed high prevalence followed by G2 (33.33%). P [6] was the main predominate type in P typing, followed by P[4] and a mixed type P[4,6]. Three strains got sequenced and deposited in Gene bank with accession numbers, KJ873130, KJ873131 and KJ854380. This study shows that RT-PCR is a promising technique for the detection of group A rotavirus. Existence of mixed strain diversity suggested us for continuous rotavirus strain surveillance for successful implementation of vaccine program in children.
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