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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(10): 1231-1242
DOI: https://doi.org/10.20546/ijcmas.2017.610.148


Prevalence and Molecular Characterization of Group a Rotavirus in Children of Punjab, India
Gurlal Singh Gill1*, Simranpreet Kaur1, Padam Nath Dwivedi2 and Jatinder Paul Singh Gill1
1School of Public Health and Zoonoses, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
2Department of Veterinary Microbiology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
*Corresponding author
Abstract:

Group A rotavirus is the leading cause of severe diarrhoea in children worldwide. So, there is need to document the prevalence of local strain(s) circulating in different regions for successful implementation of vaccine program. A total of 162 stool samples were analyzed from April 2012 to March 2013in Ludhiana and Bathinda districts of Punjab by ELISA, RNA-PAGE and RT-PCR. G and P genotyping was done by RT-PCR using genotype-specific primer sets targeting VP4 and VP7 genes. RNA PAGE, RT-PCR and ELISA showed a positive prevalence rate of 6.17%, 8.02% and 9.25%respectively. RT-PCR showed perfect agreement with other two diagnostic techniques as per Diagnostic Test Agreement. In G genotyping G1 (66.66%) showed high prevalence followed by G2 (33.33%). P [6] was the main predominate type in P typing, followed by P[4] and a mixed type P[4,6]. Three strains got sequenced and deposited in Gene bank with accession numbers, KJ873130, KJ873131 and KJ854380. This study shows that RT-PCR is a promising technique for the detection of group A rotavirus. Existence of mixed strain diversity suggested us for continuous rotavirus strain surveillance for successful implementation of vaccine program in children.


Keywords: Group A Rotavirus, Prevalence, RNA-PAGE, RT-PCR, ELISA, G and P genotyping
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How to cite this article:

Gurlal Singh Gill, Simranpreet Kaur, Padam Nath Dwivedi and Jatinder Paul Singh Gill. 2017. Prevalence and Molecular Characterization of Group a Rotavirus in Children of Punjab, India.Int.J.Curr.Microbiol.App.Sci. 6(10): 1231-1242. doi: https://doi.org/10.20546/ijcmas.2017.610.148